Strep antibody (0.1 mg)

Order number: 40070

€264.00*

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Description

This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein. It can be used in westernblots and ELISAs. It's origin is mouse and should be stored at -20°C for the long-term.
Feature
Antibody Name Strep antibody
Cell Line / Origin Mouse Hybridoma
Produced using Strep-tag II peptide conjugated to keyhole limpet hemocyanin as immunogen
Subclass IgG
Suitable for Western blot, ELISA, FACS, Immune fluorescence
Form 100 µg lyophilized from NH4CO3, contains 10 mg sucrose per mg protein
Reconstitution of lyophilized antibody Reconstitute antibody in PBS buffer at concentrations between 0.2 and 1.0 mg/ml (i.e. dissolbe with 0.5 to 0.1 PBS)
Recommended dilution for Western Blot, chromogenic detection 0.2-0.5 µg/ml antibody diluted in PBS containing 2% BSA. Use rabbit anti-mouse conjugated to peroxidase as secondary antibody.
Recommended dilution for Western Blot, chemiluminescence detection 20 ng/ml antibody diluted in PBS
Recommended dilution for FACS 10 µg/ml diluted in PBS, using e.g. goat anti mouse IgG AB conjugated to Cy2 (GE Healthcare, 1:400 dilution)
Recommended dilution for IF 10 µg/ml – 5 µg/ml antibody diluted in PBS (use secondary anti-mouse antibody)
Shipment Temperature Room tempareature
Storage Store as lyophilized powder for up to 12 months at 2-8°C. Alternatively, alinquot contents and freeze at -20°C for up to 6 months. Avoid repeated freeze-that cycles.

Disclaimer

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".