AChR (Acetylcholin receptor) protein


Proteins
Our AChr is directly purified from the is the acetylcholine receptor of Torpedo californica. It is the best example on how customized protien purification beads can be used to isolate a protein without any epitope tags. The protein was purified through agarose resin coupled to neurotoxin-3 from king cobras, a natural ligand of AChR.
AChR SDS Page
Fig. 1: Western-blot and SDS-PAGE of Achetylcholine receptor from T. californica. Anti-Nicotinic acetylcholine receptor antibody, detects bands of all subunits.
AChR QC
Fig. 2: AChR was purified via rho1d4 affinity chromatography
 

Features

Protein AChR (Acetylcholin receptor 1)
Alternative names Acteylcholine receptor subunit alpha, beta, gamma and delta
UniProt number P02712 (alpha Subunit)
P02714 (beta Subunit)
P02718 (gamma Subunit)
P02710 (gamma Subunit)
Protein class Ion channel, Ligand-gated ion channel, Receptor
Organism Torpedo Californica (electric ray)
Sequence

Full-length, alpha Subunit)


MILCSYWHV GLVLLLFSCC GLVLGSEHET RLVANLLENY NKVIRPVEHH THFVDITVGL QLIQLISVDE VNQIVETNVR LRQQWIDVRL RWNPADYGGI KKIRLPSDDV WLPDLVLYNN ADGDFAIVHM TKLLLDYTGK IMWTPPAIFK SYCEIIVTHF PFDQQNCTMK LGIWTYDGTK VSISPESDRP DLSTFMESGE WVMKDYRGWK HWVYYTCCPD TPYLDITYHF IMQRIPLYFV VNVIIPCLLF SFLTGLVFYL PTDSGEKMTL SISVLLSLTV FLLVIVELIP STSSAVPLIG KYMLFTMIFV ISSIIITVVV INTHHRSPST HTMPQWVRKI FIDTIPNVMF FSTMKRASKE KQENKIFADD IDISDISGKQ VTGEVIFQTP LIKNPDVKSA IEGVKYIAEH MKSDEESSNA AEEWKYVAMV IDHILLCVFM LICIIGTVSV FAGRLIELSQ EG

Full-length, beta Subunit


MENVRRMALG LVVMMALALS GVGASVMEDT LLSVLFETYN PKVRPAQTVG DKVTVRVGLT LTNLLILNEK IEEMTTNVFL NLAWTDYRLQ WDPAAYEGIK DLRIPSSDVW QPDIVLMNNN DGSFEITLHV NVLVQHTGAV SWQPSAIYRS SCTIKVMYFP FDWQNCTMVF KSYTYDTSEV TLQHALDAKG EREVKEIVIN KDAFTENGQW SIEHKPSRKN WRSDDPSYED VTFYLIIQRK PLFYIVYTII PCILISILAI LVFYLPPDAG EKMSLSISAL LAVTVFLLLL ADKVPETSLS VPIIIRYLMF IMILVAFSVI LSVVVLNLHH RSPNTHTMPN WIRQIFIETL PPFLWIQRPV TTPSPDSKPT IISRANDEYF IRKPAGDFVC PVDNARVAVQ PERLFSEMKW HLNGLTQPVT LPQDLKEAVE AIKYIAEQLE SASEFDDLKK DWQYVAMVAD RLFLYVFFVI CSIGTFSIFL DASHNVPPDN PFA

Full-length, gamma subunit


MVLTLLLIIC LALEVRSENE EGRLIEKLLG DYDKRIIPAK TLDHIIDVTL KLTLTNLISL NEKEEALTTN VWIEIQWNDY RLSWNTSEYE GIDLVRIPSE LLWLPDVVLE NNVDGQFEVA YYANVLVYND GSMYWLPPAI YRSTCPIAVT YFPFDWQNCS LVFRSQTYNA HEVNLQLSAE EGEAVEWIHI DPEDFTENGE WTIRHRPAKK NYNWQLTKDD TDFQEIIFFL IIQRKPLFYI INIIAPCVLI SSLVVLVYFL PAQAGGQKCT LSISVLLAQT IFLFLIAQKV PETSLNVPLI GKYLIFVMFV SMLIVMNCVI VLNVSLRTPN THSLSEKIKH LFLGFLPKYL GMQLEPSEET PEKPQPRRRS SFGIMIKAEE YILKKPRSEL MFEEQKDRHG LKRVNKMTSD IDIGTTVDLY KDLANFAPEI KSCVEACNFI AKSTKEQNDS GSENENWVLI GKVIDKACFW IALLLFSIGT LAIFLTGHFN QVPEFPFPGD PRKYVP

Full-length, dekta subunit


MGNIHFVYLLISCLYYSGCS GVNEEERLIN DLLIVNKYNK HVRPVKHNNE VVNIALSLTL SNLISLKETD ETLTSNVWMD HAWYDHRLTW NASEYSDISI LRLPPELVWI PDIVLQNNND GQYHVAYFCN VLVRPNGYVT WLPPAIFRSS CPINVLYFPF DWQNCSLKFT ALNYDANEIT MDLMTDTIDG KDYPIEWIII DPEAFTENGE WEIIHKPAKK NIYPDKFPNG TNYQDVTFYL IIRRKPLFYV INFITPCVLI SFLASLAFYL PAESGEKMST AISVLLAQAV FLLLTSQRLP ETALAVPLIG KYLMFIMSLV TGVIVNCGIV LNFHFRTPST HVLSTRVKQI FLEKLPRILH MSRADESEQP DWQNDLKLRR SSSVGYISKA QEYFNIKSRS ELMFEKQSER HGLVPRVTPR IGFGNNNENI AASDQLHDEI KSGIDSTNYI VKQIKEKNAY DEEVGNWNLV GQTIDRLSMF IITPVMVLGT IFIFVMGNFN HPPAKPFEGD PFDYSSDHPR CA
Affinity tags none
Size (excluding additional elements) alpha 52,7 kDa;
beta 56,1 kDa;
gamma 58,1 kDa;
delta 60 kDa
Expression system T. Californica (native)
Purified via Protein-specific affinity matrix using immobilized agonist ligand, size exclusion chromatography
Buffer 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 5 % glycerin; 0,1 % Triton
Purity (SDS-PAGE) >95 % as determined by SDS-PAGE
Homogeneity Size exclusion chromatography
Activity Native AChR from T.Californica, purified as functional heterotetramer binding to Neurotoxin-3 ligand
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
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AChR T. Californica AChR T. Californica
Article number: 28601
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AChR T. Californica AChR T. Californica
Native AChR from T.Californica, purified as funtional heterotetramer binding to Neurotoxin-3 ligand.
Article number: 28601
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