Pure & Active membrane proteins are challenging to obtain and keeping. Therefore we offer a selection of pure & active membrane proteins for your convience. VGT (Vesicular glutamate transporter 2) from
H. sapiens was expressend in Sf) (baculovirus) and purified with our high-quality
Rho1d4-Agarose
Fig. 1: Western-blot and SDS-PAGE of VGT
Fig. 2: VGT was purified via rho1d4 affinity chromatography
Features
| Protein | VGT (Vesicular glutamate transporter 2) |
| Alternative names | VGluT2, Differentiation-associated BNPI, Solute carrier family 17 member 6 |
| UniProt number | Q9P2U8 |
| Protein class | Organic anion transporter |
| Organism | Human (Homo sapiens) |
| Sequence | Full-length, wildtype sequence GSSG spacer (grey) and C-terminal Rho1D4 tag (TETSQVAPA)(green)
MESVKQRILA PGKEGLKNFA GKSLGQIYRV LEKKQDTGET IELTEDGKPL EVPERKAPLC DCTCFGLPRR YIIAIMSGLG FCISFGIRCN LGVAIVDMVN NSTIHRGGKV IKEKAKFNWD PETVGMIHGS FFWGYIITQI PGGYIASRLA ANRVFGAAIL LTSTLNMLIP SAARVHYGCV IFVRILQGLV EGVTYPACHG IWSKWAPPLE RSRLATTSFC GSYAGAVIAM PLAGILVQYT GWSSVFYVYG SFGMVWYMFW LLVSYESPAK HPTITDEERR YIEESIGESA NLLGAMEKFK TPWRKFFTSM PVYAIIVANF CRSWTFYLLL ISQPAYFEEV FGFEISKVGM LSAVPHLVMT IIVPIGGQIA DFLRSKQILS TTTVRKIMNC GGFGMEATLL LVVGYSHTRG VAISFLVLAV GFSGFAISGF NVNHLDIAPR YASILMGISN GVGTLSGMVC PIIVGAMTKN KSREEWQYVF LIAALVHYGG VIFYAIFASG EKQPWADPEE TSEEKCGFIH EDELDEETGD ITQNYINYGT TKSYGATTQA NGGWPSGWEK KEEFVQGEVQ DSHSYKDRVD YSGS SGTETSQVAPA
|
| Affinity tags | C-terminal Rho1D4 tag |
| Size (excluding additional elements) | 65 kDa |
| Expression system | T.ni insect cells (baculovirus expression system |
| Purified via | Rho1D4 agarose |
| Buffer | 20 mM Hepes, 10 % glycerol, 0,05% DDM, pH 7,2 |
| Purity (SDS-PAGE) | >95 % as determined by SDS-PAGE |
| Homogeneity | Size exclusion chromatography |
| Function | Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. |
