The PureCube Ni-IDA Cartridge is a chromatography column prepacked with PureCube Ni-IDA Agarose. It is available in two sizes, 1 mL bed volume and 5 mL bed volume. Please refer to the given features table to verify if the Cartridge / Column is compatible with your FPLC hardware. If this is not the case, we recommed to check out our Ni-IDA COMPACT Cartridges , that cover additional compabilities.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>50 mg/mL
Bead Ligand
Ni-IDA
Bead size
40 μm
Matrix
7.5% highly cross-linked agarose
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
6.2 x 50
11 x 80
Body material
Polypropylene
Acrylate
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
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*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
