PureCube Ni-IDA Cartridge

€34.00 *

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ML:

  • 30301
The PureCube Ni-IDA Cartridge is a chromatography column prepacked with PureCube Ni-IDA... more
Product information "PureCube Ni-IDA Cartridge"
Type: Cartridge
Bead size: 40 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: IDA
Coupled Ion: Ni2+


The PureCube Ni-IDA Cartridge is a chromatography column prepacked with PureCube Ni-IDA Agarose. It is available in two sizes, 1 mL bed volume and 5 mL bed volume. Please refer to the given features table to verify if the Cartridge / Column is compatible with your FPLC hardware. If this is not the case, we recommed to check out our Ni-IDA COMPACT Cartridges , that cover additional compabilities.

Features - Regarding Purification capabilities.

Feature  
Usage Specific binding and purification of 6x His-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >50 mg/mL
Bead Ligand Ni-IDA
Bead size 40 μm
Matrix 7.5% highly cross-linked agarose
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability 2-14

Features - Regarding Cartridge / Column itself.

Feature PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Dimensions: diameter X length (mm) 6.2 x 50 11 x 80
Body material Polypropylene Acrylate
End Plug Material Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Max. Flow Rate 6 mL/min

PDF Downloads

 
 
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
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