Protein Purification Products


Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His tagged proteins
Glutathione / GST tag Agarose & MagBeads The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific./td> Strep tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! rho1d4 tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS Activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine Activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy Activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide Activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
Top seller
PureCube Fe-NTA MagBeads PureCube Fe-NTA MagBeads
Article number: 31501-Fe
PureCube Ti-NTA MagBeads PureCube Ti-NTA MagBeads
Article number: 31501-Ti
PureCube Zr-NTA MagBeads PureCube Zr-NTA MagBeads
Article number: 31501-Zr
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
Article number: 33101
Cube MagBead Separator (1) Cube MagBead Separator (1)
Article number: 16941
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
Protein Purification Products
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PureCube Fe-NTA MagBeads PureCube Fe-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with iron (III) chloride
Article number: 31501-Fe
Sales price: From €68.00 *
PureCube Ti-NTA MagBeads PureCube Ti-NTA MagBeads
Magnetic Beads are loaded with Titanium for enrichment of phospho-peptids.
Article number: 31501-Ti
Sales price: From €68.00 *
PureCube Zr-NTA MagBeads PureCube Zr-NTA MagBeads
Magnetic Beads are loaded with Zirconium for enrichment of phospho-peptids.
Article number: 31501-Zr
Sales price: From €68.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
Our PureCube Rho1D4 agarose for 100% pure protein purification. Antibody based beads for maximum purity and specificity.
Article number: 33101
Sales price: From €232.00 *
PureCube 100 Compact Cartridge Ni-INDIGO PureCube 100 Compact Cartridge Ni-INDIGO
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml Agarose with 100 micron average bead size, equipped with a proprietary ligand for His affinity purification, loaded with nickel (II) sulfate
Article number: 75302
Sales price: From €50.00 *
Cube MagBead Separator (1) Cube MagBead Separator (1)
1 Cube MagBead Separator, for 2 microtubes of 1.5 or 2 ml volume.
Article number: 16941
Sales price: From €63.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
2 ml 50% NTA Agarose, loaded with nickel (II) sulfate
Article number: 31103
Sales price: From €100.00 *
PureCube Compact Cartridge Ni-NTA PureCube Compact Cartridge Ni-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with nickel (II) sulfate
Article number: 31302
Sales price: From €34.00 *
Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 1: PureCube Rho1D4 Agarose (1...
Rho1D4 of 2 ml 50% Rho1D4 Agarose and 5 mg lyophilized Rho1D4 peptide. ► 50% discount compared to the individual products
Article number: 33199
Sales price: €171.00 *
PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
50% Agarose of 100 µm average bead size, equipped with a proprietary ligand for His affinity purification. Loaded with nickel (II) sulfate.
Article number: 75103
Sales price: From €108.00 *
PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
1 ml 25% NTA magnetic beads, loaded with nickel (II) sulfate
Article number: 31201
Sales price: From €63.00 *
PureCube Co-NTA Agarose PureCube Co-NTA Agarose
20 ml 50% NTA Agarose, loaded with cobalt (II) chloride
Article number: 31403
Sales price: From €107.00 *
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