Protein Purification Products


Purification Resins
Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer. 

Not sure which tag to use? See our affinity tag overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His-tagged proteins
Glutathione / GST-tag Agarose & MagBeads The GST-tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST-tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific. Strep-tagged proteins
Rho1D4 / 1D4-tag Affinity Agarose & MagBeads The-Rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! Rho1d4-tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
Protein Purification Products
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PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
25% concentrated INDIGO-Ni agarose for His-tag protein purification. EDTA and DTT stable. ~100% purity for your His-tagged protein.
Article number: 75201
Sales price: From €72.00 *
Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 1: PureCube Rho1D4 Agarose (1...
Cube Biotech offers Rho1D4-tag affinity agarose & the Rho1D4 protein elution peptide together as an affordable set.
Article number: 33199
Sales price: €175.00 * €350.00 *
PureCube Ni-NTA MagBeads PureCube Ni-NTA MagBeads
Ni-NTA magnetic beads for His Tag protein purification. Ø 30 µm bead diameter. Great protein yield. 25% settled beads!
Article number: 31201
Sales price: From €65.00 *
PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
Cube Biotech's exclusive INDIGO-Ni agarose resin for His Tag protein purification. Up to 100 mg protein / 1 ml resin.
Article number: 75103
Sales price: From €112.00 *
PureCube 100 Compact Cartridge Ni-INDIGO PureCube 100 Compact Cartridge Ni-INDIGO
Our exclusive & high quality INDIGO-Ni agarose resin pre-filled in COMPACT FPLC Cartridges / Columns for His-tagged proteins!
Article number: 75302
Sales price: From €52.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Ni-NTA Agarose resin for His Tag protein purification. Ø 40 µm bead diameter. Great protein yield. For batch spin & FPLC!
Article number: 31103
Sales price: From €103.00 *
PureCube Glutathione Agarose PureCube Glutathione Agarose
Cube Biotech offers PureCube GST agarose resin for the protein Purification of GST-tagged proteins | 10 mg protein / ml resin
Article number: 32103
Sales price: From €145.00 *
PureCube Cu-NTA MagBeads PureCube Cu-NTA MagBeads
For Copper-Binding and for His-tagged protein. | Cu-NTA agarose for highest protein yield of his tagged proteins.
Article number: 31501-Cu
Sales price: From €71.00 *
PureCube Carboxy MagBeads PureCube Carboxy MagBeads
Protein purification ► Carboxygroup-carrying Magnetic Beads, for activation by NHS or weak ion-exchaning ★25% conc.★
Article number: 50201
Sales price: From €88.00 *
PureCube NHS Activated MagBeads PureCube NHS Activated MagBeads
Protein purification ► NHS-Activated Magnetic Beads, for direct coupling of biomolecules via amine groups. ★25% conc.★
Article number: 50401
Sales price: From €206.00 *
PureCube Maleimide Activated MagBeads PureCube Maleimide Activated MagBeads
Protein purification ► Maleimide-Activated Magnetic Beads, for direct coupling of biomolecules via thiol groups. ★25% conc.★
Article number: 51201
Sales price: From €235.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
Our PureCube Rho1D4 agarose for protein purification. Rho1D4antibody agarose beads for maximum purity and specificity.
Article number: 33101
Sales price: From €239.00 *
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