The Intelligent Infusions Speakers

A Set Of Scientific Masterclasses

Our scientific masterclass series enables researchers from around the world to present their latest results and findings to a global audience. This series provides a unique platform for scientists to share their work, connect with others in their field, and receive feedback from experts and peers. As a viewer, you will benefit from interactive features such as a live Q&A and the ability to watch all talks again later on demand.

Overview of the upcoming sessions

Portrait of Michael Erkelenz
Dr. Michael Erkelenz
Cube Biotech
Custom Functionalized Particles: From Micrometer-Scale Agarose to Nanometer-Scale Noble Metals
April 25th 2024 | 16:00 - 16:45 CET

KEY TAKEAWAYS

  • Functionalized particles have played an integral role across diverse sectors, spanning from pioneering scientific endeavors to large-scale biotechnological production. These particles encompass a spectrum of base materials, ranging from agarose to noble metals, each endowed with distinct physicochemical properties tailored to specific applications.
  • The functionality of these particles may either be inherent to the material itself, exemplified by gold nanoparticles showcasing unique optical properties manifested in localized surface plasmon resonance, or can be augmented through molecular functionalization of foundational substrates, such as agarose.
  • Introducing Cube Biotech's latest functional particle service, we address the comprehensive spectrum of needs, extending from material selection to functionalization, and from proof-of-concept to the industrial-scale production of bespoke (nano)particle constructs.

HIS RESEARCH

Dr. Michael Erkelenz, currently employed as a chemist at Cube Biotech, has an academic and professional background in physical chemistry, with a specialization in nano-biophotonics.
Commencing in October 2017, he initiated his doctoral research at the University of Duisburg-Essen, focusing on pioneering developments in advanced plasmonic DNA nanostructures within the NanoBioPhotonics field. Throughout this period, he conducted extensive work in the synthesis, functionalization, and characterization of gold nanoparticles for applications in immune-based theranostics, culminating in December 2021.
His academic journey commenced with a Bachelor of Science in Biology, General, from Heinrich-Heine-Universität Düsseldorf, graduating in 2015. This followed the successful completion of a Master of Science in Medical Biology at the the University of Duisburg-Essen from 2015 to 2017. Finally, he obtained his PhD in Physical Chemistry from the same institution between 2017 and 2021.
Throughout his academic pursuits, he actively participated in various academic societies and research initiatives, notably contributing as a member of Collaborative Research Center.
Armed with a robust understanding of physical chemistry and extensive expertise in nanoparticle synthesis and characterization, Michael Erkelenz remains dedicated to advancing biofunctionalized particle technologies at Cube Biotech.
KEYWORDS: Nanoparticles, Particles, Functionalization, Customize, Single Molecular, Diagnostics, Noble Metals,
Portrait of Renee Chow
Dr. Renee Chow
Monash University & ARMI
TBA
May 29th 2024

KEY TAKEAWAYS

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HER RESEARCH

More information is coming soon...
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Rewatch past sessions

Portrait of Michael Erkelenz and Philipp Hanisch
Dr. Michael Erkelenz
Dr. Philipp Hanisch
Cube Biotech
Unveiling the Membrane Protein Puzzle: Exploring Copolymer Solubilization

KEY TAKEAWAYS

  • Challenges in working with membrane proteins include integration, stability, expression, purification, structural heterogeneity, functional assays, and crystallization.
  • Copolymers like SMA, DIBMA, AASTYs, and Ultrasolute Amphipol offer solutions by aiding in extraction, solubilization, stabilization, purification, structural studies, functional assays, and drug discovery related to membrane proteins.
  • Solubilization with copolymers involves formation of lipid bilayer nanodiscs encapsulating membrane proteins in a native-like environment, overcoming challenges of traditional methods and offering versatile applications in research and therapeutic development.

THEIR RESEARCH

Philipp Hanisch is an accomplished scientist with extensive expertise in membrane protein expression, purification, cell biology, microscopy, and product development. Currently serving as the Head of Laboratory at Cube Biotech GmbH in Monheim am Rhein, Germany, Philipp leads a dynamic team in driving innovative solutions in protein science. Prior to his current role, he held positions as a Senior Scientist and Postdoctoral Researcher, contributing significantly to the advancement of Cubes copolymer service platform.
Philipp holds a Doctor rer nat. (Ph.D.) in Biology from Heinrich-Heine-Universität Düsseldorf, where he graduated magna cum laude from the CRC1208 - Identity and Dynamics of Membrane Systems. Since his master thesis he worked on chlamydial polymorphic membrane proteins (Pmps), CPn0677 and the Lipid Internalization Promoting Protein (LipP), biochemically analysing the targets and identifying human interaction partners.
In addition to his scientific pursuits, Philipp has also served as a Freelance Consultant for Orientation and Personal Development, showcasing his versatility and dedication to personal growth and mentorship.

Dr. Michael Erkelenz, currently employed as a chemist at Cube Biotech, has an academic and professional background in physical chemistry, with a specialization in nano-biophotonics.
Commencing in October 2017, he initiated his doctoral research at the University of Duisburg-Essen, focusing on pioneering developments in advanced plasmonic DNA nanostructures within the NanoBioPhotonics field. Throughout this period, he conducted extensive work in the synthesis, functionalization, and characterization of gold nanoparticles for applications in immune-based theranostics, culminating in December 2021.
His academic journey commenced with a Bachelor of Science in Biology, General, from Heinrich-Heine-Universität Düsseldorf, graduating in 2015. This followed the successful completion of a Master of Science in Medical Biology at the the University of Duisburg-Essen from 2015 to 2017. Finally, he obtained his PhD in Physical Chemistry from the same institution between 2017 and 2021.
Throughout his academic pursuits, he actively participated in various academic societies and research initiatives, notably contributing as a member of Collaborative Research Center.
Armed with a robust understanding of physical chemistry and extensive expertise in nanoparticle synthesis and characterization, Michael Erkelenz remains dedicated to advancing biofunctionalized particle technologies at Cube Biotech.
KEYWORDS: Nanodiscs, Membrane Proteins, Protein, Synthetic Polymers, Basic Research, Proteomics, Polymer Technology, Laboratory Technology
Portrait of Gregory Dodge
Greg J. Dodge, PhD
MIT
Membrane Proteins & Glycobiology
SMALPs as a platform to study bacterial membrane proteins in a native-like environment

KEY TAKEAWAYS

  • SMALPs represent an excellent alternative to classical detergent-based strategies for challenging bacterial membrane proteins.
  • Optimization of expression and purification strategies is critical to obtain the highest-quality material for structural studies.
  • In our studies, by stabilizing the monotopic phosphoglycosyl transferase WbaP in SMALP, we were able to characterize this important protein in a near-native environment using a variety of techniques, including SEC-MALs, mass photometry, crosslinking mass spectrometry, native mass spectrometry, and CryoEM.
  • Adapting new technologies as NanoDSF has enabled us to develop high-throughput methods to rapidly screen different polymers to identify optimal conditions for solubilization. This workflow is amenable to automation, and we anticipate that it will greatly simplify the otherwise empirical polymer screening steps.

HIS RESEARCH

Greg grew up in a small town in the finger lakes region of western New York. After obtaining his bachelor’s degree in Biochemistry from Rochester Institute of Technology, he began his PhD studies at the University of Michigan. At Michigan, he joined the laboratory of Prof. Janet Smith, where he studied dehydratase domains from prokaryotic polyketide synthase and fatty acid synthase using X-ray crystallography. In 2018, he joined the laboratory of Prof. Barbara Imperiali at MIT, where he was awarded a Ruth L. Kirschstein F32 fellowship for the study of phosphoglycosyl transferases from glycoconjugate assembly pathways in human pathogens. Outside of lab, Greg enjoys traveling, hiking with his wife and dog, and tinkering with any and all kinds of technology.
Portrait of Luis Real Hernandez
Luis Real Hernandez
University of Virginia
Membrane Biophysics
Lipid Bilayer Perturbations in Membrane Nanoparticles

KEY TAKEAWAYS

  • Copolymer solubilization of membranes directly extracts membrane lipids into membrane nanoparticles
  • While preserving membrane protein function in membrane nanoparticles is important, so is preserving native membrane lipid properties
  • Lipid packing sensitive dyes report differences in the lipid properties of membrane nanoparticles compared to intact membranes
  • Across different lipid compositions, the biophysical properties of intact membranes are not inherently preserved in copolymer-stabilized nanoparticles

HIS RESEARCH

Luis Real Hernandez obtained his master’s degree at The Ohio State University where he focused on analyzing the interfacial behavior of dairy polar lipids. He is currently a biophysics PhD candidate at the University of Virginia studying the lipid environment selectivity of membrane proteins. Currently, he is developing methods to determine the preferred surrounding lipids of membrane proteins using copolymeric nanodiscs.
RECENT PUBLICATION: Lipid packing is disrupted in copolymeric nanodiscs compared with intact membranes
KEYWORDS: Fluorescence spectroscopy; Lipid packing; Lipid composition; Membrane structure; Biophysical properties
Portrait of Jonas Bossart
Jonas Bossart
Empa, ETH Zürich
Quantitative Proteomics & Nanomedicine
Characterization of macrophage phenotypic states and cellular responses to nanomedicines

KEY TAKEAWAYS

  • Pro-inflammatory phenotypes show high activity of p38, JNK and JAK kinases, while PAK2 and PKCα kinases are important regulators of immunosuppressive macrophages
  • Proteomic signatures of in vitro differentiated macrophages enable the separation of pro-inflammatory populations in a clinical setting
  • Macrophages internalize iron sucrose as complete nanoparticles and dissociated iron ions
  • Exposure to iron sucrose induces signs of M2 polarization loss and mechanisms to prevent ferroptosis
  • Iron-carbohydrate complexes with a more dynamic interaction are internalized and biodegraded faster

HIS RESEARCH

Jonas Bossarts research is focused on quantitative proteomics of human immune cells for defining their roles in disease development and treatment responses. Currently, he is investigating the phenotypic landscape of macrophages and their response to widely used iron-carbohydrate complex nanomedicines.
RECENT PUBLICATION: Uncovering the dynamics of cellular responses induced by iron-carbohydrate complexes in human macrophages using quantitative proteomics and phosphoproteomics
KEYWORDS: Quantitative proteomics, Phosphoproteomics, Macrophages, Polarization, Iron-carbohydrate complexes
Portrait of Dr. Merino
DR. FELIPE MERINO
Cube Biotech, Head of Cryo-EM
Structural Biology & Biochemistry
Synthetic Co-Polymer Nanodisc Enabling Near-Native Membrane Protein Assays

KEY TAKEAWAYS

  • The key bottleneck of membrane protein research is finding solubilization and stabilization conditions that preserve the native structure and function of the protein
  • Synthetic co-polymers — such as styrene-maleic anhydride (SMA), di-isobutylene maleic acid (DIBMA), acrylic acid-co-styrene (AASTY) and UltrasoluteTM Amphipols — can extract membrane proteins and their surrounding lipids directly from the membrane, self-assembling into a so-called native nanodisc
  • State-of-the-art copolymers have solubilization efficiencies that rival those of detergents, while simultaneously providing superior stabilization and preserving native function
  • The power of synthetic polymers can be leveraged to lyse eukaryotic cells

HIS RESEARCH

Dr. Merino obtained his PhD at the Max Planck Institute for Molecular Biomedicine Münster working on macromolecular modelling and simulation. He later became an expert in electron cryomicroscopy through his postdoctoral training at the Max Planck Institute for Molecular Physiology in Dortmund and as a project leader at the Max Planck Institute for Biology in Tübingen. His combined expertise in atomic modeling and cryo-EM has provided him with a unique skill set allowing him to tackle challenging structural biology problems, such as those typically presented by membrane proteins.
RECENT PUBLICATION: The bigger picture: global analysis of solubilization performance of classical detergents versus new synthetic polymers utilizing shotgun proteomics
KEYWORDS: Protein, Synthetic Polymers, Basic Research, Proteomics, Polymer Technology, Laboratory Technology
Portrait of Prof. Klumperman
PROF. BERT KLUMPERMAN
Stellenbosch University and Nanosene (Pty) Ltd., South Africa
Biomedicine & Biochemistry
Unleashing the potential of 2nd generation SMA copolymers

KEY TAKEAWAYS

  • Differences between conventional and controlled polymerization methods
  • Shortcomings of conventional polymerization method
  • Benefits of controlled polymerization methods
  • Utilization of controlled polymerization for added functionality

HIS RESEARCH

Prof. Klumperman's current research interests are reversible deactivation radical polymerization (RDRP), the SMALP technology and biomedical applications of polymers in general. In 2017 he was elected a Fellow of the African Academy of Sciences, and in 2018 he received the John FW Herschel medal of the Royal Society of South Africa. He has served as Vice-President of the Royal Society of South Africa from 2021. Since 2022 Bert Klumperman is the Chief Technology Officer of a spin-off company, Nanosene (Pty) Ltd, established on the basis of research in his academic group.
Portrait of Dr. Swainsbury
DR. DAVID SWAINSBURY
Faculty of Science, University of East Anglia, GB
Biochemistry & Microbiology
Photosynthetic membrane proteins demonstrate the utility of SMA copolymers

KEY TAKEAWAYS

  • Styrene maleic acid (SMA) copolymers create lipid nanodiscs directly from biological membranes, avoiding the need to use detergents.
  • Work on model membrane proteins from the photosynthetic bacterium Rba. sphaeroides reveals that native structures and functions are retained in SMA nanodiscs
  • Membrane proteins purified in SMA nanodiscs are amenable to biochemical characterisation, and structural determination by cryo-EM.

HIS RESEARCH

Dr Swainsbury’s research centres on structural and functional studies of photosynthetic protein complexes to understand the molecular mechanisms that convert light energy into useful chemical forms. The model membrane proteins that are central to his research have been invaluable to understand the mechanisms by which styrene maleic acid (SMA) copolymers produce lipid nanodiscs containing membrane protein complexes directly from cell membranes. His publications show how SMA retains the biochemical properties that are often lost when removing the protein from its membrane with detergents, how SMA can act as a probe for protein in lipid-rich or protein-rich domains, and most recently how SMA allows the structural characterisation of protein complexes that are labile in detergents to be determined by cryogenic electron microscopy.
RECENT PUBLICATION: Cryo-EM structure of the four-subunit Rhodobacter sphaeroides cytochrome bc1 complex in styrene maleic acid nanodiscs
KEYWORDS: Cryo-EM; Protein engineering; Protein design; Protein purification; Spectroscopy; Enzymology
Portrait of Dr. Nadezhdin
DR. KIRILL NADEZHDIN
Columbia University Irving Medical Center, New York
Biophysics & Biochemistry
Structural mechanisms of TRPM7 activation and inhibition

KEY TAKEAWAYS

  • Cryogenic electron microscopy (cryo-EM) enables the resolution of membrane protein structures in complex with both natural and synthetic ligands.
  • In most cases, protein biochemistry is a limiting factor for structure elucidation using cryo-EM.
  • Elucidating the structures of ligand-bound proteins helps to understand the molecular mechanisms of their action.

HIS RESEARCH

Kirill Nadezhdin completed his undergraduate studies at Moscow Institute of Physics and Technology, where he focused on mathematics and physics with applications to biological systems. He earned his PhD in Biophysics from Lomonosov Moscow State University while researching the amyloid precursor protein associated with Alzheimer's disease. Following that, he joined Columbia University in the City of New York as a postdoctoral research scientist, where he specializes in studying the structures of ion channels using cryogenic electron microscopy (cryo-EM).
RECENT PUBLICATION: Structural mechanisms of TRPM7 activation and inhibition
KEYWORDS: Cryoelectron microscopy; Transient receptor potential channels; Cellular sensors
Portrait of Dr. Damiati
DR. SAMAR DAMIATI
Department of Chemistry, University of Sharjah, U.A.E.
Nanobiotechnology
Building an artificial cell membrane from scratch

KEY TAKEAWAYS

  • Bottom-up synthetic biology allows us to use the basic elements (non-living components) of biological origins to construct artificial life de novo
  • Biomimetic membranes can be constructed using easily generated system composed of proteins and lipids to form vesicles, mono- or bilayer membranes
  • Lipid membrane-based biosensors can be developed and characterized as acoustic biosensors for cell mimicry which enable in vitro investigation of biomolecular interactions
  • Using cell-free protein expression system allows production of rightly folded membrane proteins from exogeneous mRNA or DNA, which can be directly incorporated into artificial cell models

HER RESEARCH

Damiatis research is focused on bottom-up synthetic biology to construct artificial cell membranes that mimic the features and behavior of biological ones. Currently, she uses microfluidic technology to construct artificial cell models and drug delivery systems.
RECENT PUBLICATION: Albumin-bound nanodiscs as delivery vehicle candidates: Development and characterization
KEYWORDS: Synthetic bioarchitectures; Nanoparticles; Drug delivery; Nanomedicine
Portrait of Toman Borteçen
TOMAN BORTEÇEN
German Cancer Research Center (DKFZ)
Proteomics
A semi-automated workflow for the quantitative analysis of the newly synthesized proteome

KEY TAKEAWAYS

  • Quantitative analysis of newly synthesized proteins provides insights into cellular responses to signalling events on rapid time scales
  • Through the use of novel magnetic alkyne beads, the enrichment of newly synthesized proteins can be performed with a semi-automated protocol using 10-fold lower input.
  • Multiplexed data-independent-acquisition (plexDIA) LC-MS analysis of heavy isotope labelled samples, greatly increases protein identifications while preserving high quantitative accuracy.
  • Using the integrated workflow, well characterised changes in protein synthesis, can be quantified after only 2h treatments of cells.

HIS RESEARCH

Toman Borteçens research is focused on the development of quantitative proteomic methods and their application for the study of protein synthesis. The main goal of his ongoing PhD project is investigating the regulatory mechanisms of mRNA translation in cancer cells.
RECENT PUBLICATION: An integrated workflow for quantitative analysis of the newly synthesized proteome
KEYWORDS: Proteomics; Automation; Click-chemistry; Cell signalling
Portrait of Dr. Schönberger
DR. NORA SCHÖNBERGER
Helmholtz Institute Freiberg for Resource Technology
Customized Beads
Biomolecular recycling - Application of phage surface display for the development of tailored peptides for the recovery of gallium and other high-tech metals

KEY TAKEAWAYS

  • Phage surface display technology allows us to develop specialized peptides by the complementary assembly to molecular architectures.
  • These peptides can selectively bind metal-containing particles or metal ions.
  • Immobilization of the peptides on support materials results in stable biocomposites, with high affinity for a specific target material.
  • Peptide-based biocomposites can be used in innovative recycling and separation processes.

HER RESEARCH

Schönbergers research is focused on the development of peptide-based biomolecules that can selectively recognize and separate hypocritical materials. Currently, she is interested in the selective recognition of plastic surfaces by different peptides for the recognition of nano/microplastics as well as the bio-functionalization of plastics.
RECENT PUBLICATION: Chromatopanning for the identification of gallium binding peptides
KEYWORDS: Phage display; Gallium; Peptides; Chromatopanning
Portrait of Dr. Leutert
DR. MARIO LEUTERT
ETH Zürich
Phosphoproteomics
Multidimensional proteomics deciphers regulatory protein networks in cellular signaling and aging

KEY TAKEAWAYS

  • Novel workflows facilitate large-scale mass spectrometry-based (phospho-) proteomics for systems biology.
  • Profiling phosphoproteomic responses to 101 environmental and chemical perturbations reveals functional organization principles of the phosphorylation signaling network.
  • Quantitative proteomics of protein abundance, turnover, thermal stability, and phosphorylation across the replicative lifespan of S. cerviseae identifies thousands of age-affected molecular events.
  • Integrated analysis of age-dependent proteome remodeling elucidates molecular trajectories of cellular aging and rejuvenation.

HIS RESEARCH

The research of Leutert focuses on developing and applying experimental and computational mass spectrometry-based proteomic technologies to perform high-dimensional quantitative experiments for understanding biological processes in aging and disease.
RECENT PUBLICATION: Multidimensional proteomics identifies molecular trajectories of cellular aging and rejuvenation
KEYWORDS: Proteomics; Cellular aging; S. cerevisiae; Mechanisms of aging
Portrait of Dr. Hanisch
DR. PHILIPP HANISCH
Head of Laboratory - Protein Service at Cube Biotech GmbH
Copolymer Nanodiscs
Solving ongoing difficulties in membrane protein purification using established and brand new copolymers
Dr. Philipp T. Hanisch talks about how synthetic copolymers, such as AASTY or DIBMA are used for membrane protein stabilization. Learn more about the advantages this method has over existing approaches like detergents. Furthermore, find out what polymer fits your purpose best. So, if you are planning a project regarding a membrane protein this webinar is for you!
KEYWORDS: Synthetic copolymers; Nanodiscs; Detergents; Membrane proteins

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