Our high quality Ni-IDA agarose resin beads are also available in XL size. They may come with decreased protein yield compared to 40 µm Ni-IDA counter part , but they make up in flow rate. This comes especially handy when working with very viscous cell lysates.
Features
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>20 mg/mL
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity
Delivered as a 50 % suspension
Bead size
400 μm
Bead Ligand
Ni-IDA
Required equipment
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
50 mL centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
