The Rho1D4 peptide is a 9 amino acid long chain whose purpose is the elution of proteins that are bound to Rho1D4 affinity beads during the corresponding protein purification procedure. The main principle here is competetive binding. Since the small Rho1D4 peptides can be added in excessive amounts into the elution buffer they outcompete the bound Rho1D4-tagged proteins by binding to the affinity beads instead. This way the proteins are eluted and the final step of the purification is done.
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Features
Usage
Specifity
Affinity to Rho1D4 affinity beads
Sequence
Ac_TETSQVAPA_ NH2
Amount
5 mg, lyophilized
Storage, short term
Store lyophilized and reconstituted peptide at 4°C
Storage, long term
Store lyophilized and reconstituted peptide at-20°C
Reconstitution and Dilution
Reconstitute Rho1D4 peptide in 0.53 mL ddH20 to obtain a 10 mM stock solution. Dilute stock solution in elution buffer directly before use as follows:
Final Rho1D4 peptide concentration in elution buffer
