Rho1D4 peptide

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The Rho1D4 peptide is a 9 amino acid long chain whose purpose is the elution of proteins... more

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Product information "Rho1D4 peptide"

The Rho1D4 peptide is a 9 amino acid long chain whose purpose is the elution of proteins that are bound to Rho1D4 affinity beads during the corresponding protein purification procedure.
The main priciple here is competetive binding. Since the small Rho1D4 peptides can be added in excessive amounts into the elution buffer they outcompete the bound Rho1D4-tagged proteins by binding to the affinity beads instead. This way the proteins are eluted and the final step of the purification is done.

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Features

Usage
Specifity	Affinity to Rho1D4 affinity beads
Sequence	Ac_TETSQVAPA_ NH₂
Amount	5 mg, lyophilized
Storage, short term	Store lyophilized and reconstituted peptide at 4°C
Storage, long term	Store lyophilized and reconstituted peptide at-20°C

Reconstitution and Dilution

Reconstitute Rho1D4 peptide in 0.53 mL ddH₂0 to obtain a 10 mM stock solution. Dilute stock solution in elution buffer directly before use as follows:

Final Rho1D4 peptide concentration in elution buffer	Dilution of stock solution	Final volume of elution buffer
200 µM	1:50	26.45 mL
400 µM	1:25	13.26 mL
500 µM	1:20	10.58 mL
800 µM	1:12.5	6.62 mL
1000 µM	1:10	5.29 mL

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