Channelrhodopsin-1 (Ca)

Order number: 28941

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Description

Channelrhodopsin 1 is a 7-transmembrane protein that acts as a light-driven cation channel in eukaryotic green algae. Due to its covalently bound colored ligand, it is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide channelrhodopsin 1 of high purity, purified from yeast as a His-tagged protein.
Feature
Alternative names Channelrhodopsin 1 (CaChR1)
UniProt Number G8HKA1
Protein class 7-transmembrane protein
Organism Chloromonas augustae (Chlamydomonas augustae)
Sequence, explained wild-type sequence, N-terminal membrane-spanning domain (362 of 715 aa), 10x His-tag
Sequence, One-Letter Code MDTLAWVARE LLSTAHDATP ATATPSTDHS TPSTDHGSGE TFNVTITIGG GHHGGHAGPV DNSIVIGGID GWIAIPAGDC YCAGWYVSHG SSFEATFAHV CQWSIFAVCI LSLLWYAWQY WKATCGWEEV YVCCIELVFI CFELYHEFDS PCSLYLSTAN IVNWLRYSEW LLCCPVILIH LSNVTGLSDD YGRRTMGLLV SDIATIVFGI TAAMLVSWPK IIFYLLGFTM CCYTFYLAAK VLIESFHQVP KGICRHLVKA MAITYYVGWS FFPLIFLFGQ SGFKKISPYA DVIASSFGDL ISKNMFGLLG HFLRVKIHEH ILKHGDIRKT THLRIAGEEK EVETFVEEED EDHHHHHHHH HH
Affinity tags His-tag (C-terminus)
Expression Host Pichia pastoris (yeast)
Size 362 amino acids; 40,661 Da
Buffer composition 100 mM NaCl, 20 mM HEPES pH 7.4, 0.03% dodecyl maltoside (DDM)
Purity >98%, determined via SDS-PAGE (Figure 1)
Purified via PureCube Ni-NTA Agarose
Absorbance Extinction coefficient at 518 nm: 36,000 M-1 cm-1..
Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 518 nm = 2.2 (additional vibronic band at 480 nm)
Function Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven cation channel
Literature references
  1. Hou, Sing‐Yi, et al. "Diversity of Chlamydomonas channelrhodopsins." Photochemistry and photobiology 88.1 (2012): 119-128.
  2. Muders, Vera, et al. "Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1." FEBS letters 588.14 (2014): 2301-2306.

Lab Results

Channelodopsin (Np) SDS-PAGE
Figure 1: SDS-PAGE of channelrhodopsin 1 from C. augustae.
Channelodopsin absoprtion spectrum measured by Cube Biotech
Figure 2: Absorbance spectrum of channelrhodopsin 1 from C. augustae.

FAQ

Can I get the datasheet for the Channelodopsin protein?

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