Channelrhodopsin 1 (Ca)


Proteins
Channelrhodopsin 1 is a 7-transmembrane protein that acts as a light-driven cation channel in eukaryotic green algae. Due to its covalently bound colored ligand, it is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide channelrhodopsin 1 of high purity, purified from yeast as a his-tagged protein.

channelrodopsin1_SDS-Page
Figure 1: SDS-PAGE of channelrhodopsin 1 from C. augustae.
channelrodpsin1_absorbance
Figure 2: Absorbance spectrum of channelrhodopsin 1 from C. augustae.

 

Features

Protein Channelrhodopsin 1 (CaChR1)
UniProt number G8HKA1
Protein class 7-transmembrane protein
Organism Chloromonas augustae (Chlamydomonas augustae)
Sequence

wild-type sequence, N-terminal membrane-spanning domain (362 of 715 aa), 10x His-tag (red)


MDTLAWVARE LLSTAHDATP ATATPSTDHS TPSTDHGSGE TFNVTITIGG GHHGGHAGPV DNSIVIGGID GWIAIPAGDC YCAGWYVSHG SSFEATFAHV CQWSIFAVCI LSLLWYAWQY WKATCGWEEV YVCCIELVFI CFELYHEFDS PCSLYLSTAN IVNWLRYSEW LLCCPVILIH LSNVTGLSDD YGRRTMGLLV SDIATIVFGI TAAMLVSWPK IIFYLLGFTM CCYTFYLAAK VLIESFHQVP KGICRHLVKA MAITYYVGWS FFPLIFLFGQ SGFKKISPYA DVIASSFGDL ISKNMFGLLG HFLRVKIHEH ILKHGDIRKT THLRIAGEEK EVETFVEEED EDHHHHHHHH HH

Affinity tags His-tag (C-terminus)
Size 362 amino acids; 40,661 Da
Expression system Pichia pastoris (yeast)
Purified via PureCube Ni-NTA Agarose
Buffer 100 mM NaCl, 20 mM HEPES pH 7.4, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 518 nm: 36,000 M-1 cm-1.
Absorbance Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 518 nm = 2.2 (additional vibronic band at 480 nm)
Function Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven cation channel
Literature references
  1. Hou, Sing‐Yi, et al. "Diversity of Chlamydomonas channelrhodopsins." Photochemistry and photobiology 88.1 (2012): 119-128.
  2. Muders, Vera, et al. "Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1." FEBS letters 588.14 (2014): 2301-2306.
Proteins
Channelrhodopsin 1 is a 7-transmembrane protein that acts as a light-driven cation channel in eukaryotic green algae. Due to its covalently bound colored ligand, it is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide channelrhodopsin 1 of high purity, purified from yeast as a his-tagged protein.

channelrodopsin1_SDS-Page
Figure 1: SDS-PAGE of channelrhodopsin 1 from C. augustae.
channelrodpsin1_absorbance
Figure 2: Absorbance spectrum of channelrhodopsin 1 from C. augustae.

 

Features

Protein Channelrhodopsin 1 (CaChR1)
UniProt number G8HKA1
Protein class 7-transmembrane protein
Organism Chloromonas augustae (Chlamydomonas augustae)
Sequence

wild-type sequence, N-terminal membrane-spanning domain (362 of 715 aa), 10x His-tag (red)


MDTLAWVARE LLSTAHDATP ATATPSTDHS TPSTDHGSGE TFNVTITIGG GHHGGHAGPV DNSIVIGGID GWIAIPAGDC YCAGWYVSHG SSFEATFAHV CQWSIFAVCI LSLLWYAWQY WKATCGWEEV YVCCIELVFI CFELYHEFDS PCSLYLSTAN IVNWLRYSEW LLCCPVILIH LSNVTGLSDD YGRRTMGLLV SDIATIVFGI TAAMLVSWPK IIFYLLGFTM CCYTFYLAAK VLIESFHQVP KGICRHLVKA MAITYYVGWS FFPLIFLFGQ SGFKKISPYA DVIASSFGDL ISKNMFGLLG HFLRVKIHEH ILKHGDIRKT THLRIAGEEK EVETFVEEED EDHHHHHHHH HH

Affinity tags His-tag (C-terminus)
Size 362 amino acids; 40,661 Da
Expression system Pichia pastoris (yeast)
Purified via PureCube Ni-NTA Agarose
Buffer 100 mM NaCl, 20 mM HEPES pH 7.4, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 518 nm: 36,000 M-1 cm-1.
Absorbance Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 518 nm = 2.2 (additional vibronic band at 480 nm)
Function Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven cation channel
Literature references
  1. Hou, Sing‐Yi, et al. "Diversity of Chlamydomonas channelrhodopsins." Photochemistry and photobiology 88.1 (2012): 119-128.
  2. Muders, Vera, et al. "Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1." FEBS letters 588.14 (2014): 2301-2306.
Channelrhodopsin 1 (Ca)
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Channelopsin 1 (Chloromonas augustae, 100 µg) Channelopsin 1 (Chloromonas augustae, 100 µg)
Phototaxis involded, membrane bound protein Channelopsin 1 from Chloromonas augustae. ► Pure and ready for use.
Article number: 28941
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