Sensory Rhodopsin-2 (Hs)
Order number: 28921
Description
Sensory rhodopsin-2 (SR-2) is a 7-transmembrane helice protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E.coli as a His-tagged protein.
Datasheets
Feature | |
---|---|
Alternative names | Sensory rhodopsin-2 (SR-2, NpSRII) |
UniProt Number | P71411 |
Protein class | 7-transmembrane protein |
Organism | Halobacterium salinarum S9 |
Sequence, explained | wild-type sequence, full-length, 6x His-tag |
Sequence, One-Letter Code | MALTTWFWVG AVGMLAGTVL PIRDCIRHPS HRRYDLVLAG ITGLAAIAYT TMGLGITATT VGDRTVYLAR YIDWLVTTPL IVLYLAMLAR PGHRTSAWLL AADVFVIAAG IAAALTTGVQ RWLFFAVGAA GYAALLYGLL GTLPRALGDD PRVRSLFVTL RNITVVLWTL YPVVWLLSPA GIGILQTEMY TIVVVYLDFI SKVAFVAFAV LGADAVSRLV AADAAAPATA EPTPDGD HHHHHH |
Affinity tags | His-tag (C-terminus) |
Expression Host | E.coli |
Size | 243 amino acids; 26,103 Da |
Buffer composition | 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM) |
Purity | >98%, determined via SDS-PAGE (Figure 1) |
Purified via | PureCube Ni-NTA Agarose |
Absorbance | Extinction coefficient at 497 nm: 48,000 M-1 cm-1.. Extinction coefficient at 590 nm: 54,000 M-1 cm-1. |
Activity | Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 497 nm = 1.7(additional vibronic bands at 460 nm and 420 nm) |
Function | Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor |
Literature references |
Lab Results
FAQ
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