Protocols & Datasheets
Our protocols for protein expression, purification and buffer optimization are constantly revised and updated. They are available for free download.
General Protocols
Protocols for protein purification and detection
| All His affinity resins | GST affinity | Strep affinity | Rho1D4 affinity | Activated | |
|---|---|---|---|---|---|
| Purification using Affinity Agarose | native | native | native | Amine Carboxy Epoxy Maleimide NHS Thiol | |
| Purification using 1-step Batch Midi Plus Columns | native | native | native | Carboxy NHS | |
| Purification using 1-step Batch Mini Columns | native | native | native | - | |
| Purification using MagBeads | native | native | native | Amine Carboxy Epoxy Maleimide NHS Thiol | |
| Regeneration | Cleaning and Regeneration | Cleaning and Regeneration | Cleaning and Regeneration | Cleaning and Regeneration | - |
| Special applications | Nickel loading of IMAC Agarose Al/Co/Cu/Fe/Zn loading of IMAC Agarose Nickel loading of IMAC MagBeads AL/Co/Cu/Fe/ZN loading of IMAC MagBeads |
Pulldown using MagBeads | - | - | - |
| Western Blot detection | Detection | Detection | - | Detection | - |
Nanodisc protocols
Membrane scaffold protein
| MSP1D1 His-tagged | MSP1D1 | MSP1D1-deltaH5 His-tagged | MSP1D1-deltaH5 | MSP1E3D1 His-tagged | |
|---|---|---|---|---|---|
| Reconstitution of detergent- solubilized membrane proteins using MSPs UPDATED 01/16 |
DMPC POPC | DMPC POPC | DMPC POPC | DMPC POPC | DMPC POPC |
| Nanodisc assembly for cell-free expression reactions - using Nanodisc Assembly Kits UPDATED 02/16 |
DMPC POPC | DMPC POPC | DMPC POPC | DMPC POPC | DMPC POPC |
Cube Datasheets
| Other Affinity Agaroses | Other Affinity MagBeads | Other Affinity Cartridges |
|---|---|---|
| Rho1D4 | Rho1D4 | Rho1D4 |
| PureCube Rho1D4 Agarose | PureCube Rho1D4 MagBeads | PureCube Rho1D4 Cartridges |
| PureCube Compact Cartridge Rho1D4 | ||
| Glutathione | Glutathione | Glutathione |
| PureCube Glutathione Agarose | PureCube Glutathione MagBeads | PureCube Glutathione Cartridges |
| PureCube Glutathione Agarose XL | PureCube Compact Cartridge Glutathione | |
| StrepTactin | StrepTactin | StrepTactin |
| PureCube HiCap StrepTactin Agarose | PureCube HiCap StrepTactin MagBeads | PureCube HiCap StrepTactin Cartridges |
| PureCube Compact Cartridge HiCap StrepTactin |
| Activated Agaroses | Activated MagBeads | Other Agaroses & MagBeads |
|---|---|---|
| Unloaded IDA and NTA | ||
| PureCube Amine Agarose | PureCube Amine MagBeads | PureCube IDA Agarose |
| PureCube Carboxy Agarose | Pure Cube Carboxy MagBeads | PureCube IDA MagBeads |
| PureCube Epoxy Agarose | PureCube Epoxy MagBeads | PureCube NTA Agarose |
| PureCube Maleimide Agarose | PureCube Maleimide MagBeads | PureCube 100 NTA Agarose |
| PureCube NHS Agarose | PureCube NHS MagBeads | PureCube NTA MagBeads |
| PureCube Thiol Agarose | PureCube Thiol MagBeads | |
| Hydrophobic Interaction Matrices | ||
| PureCube Butyl Agarose | ||
| PureCube Octyl Agarose | ||
| PureCube Phenyl Agarose | ||
| Ion Exchange Matrices | ||
| PureCube CM Agarose |
| Purified Proteins | Protein Crystallization | Accessories |
|---|---|---|
| Human GPCRs | CubeCrystal Plates | Columns to fill |
| GPBAR1/TGR5 | PureCube 1-step batch Mini | |
| OPRM1 in nanodisc | PureCube 1-step batch Midi Plus | |
| GLP1R | PureCube Cartridge, 1 mL | |
| NTSR1 | PureCube Compact Cartridge, 1 mL | |
| PureCube Cartridge, 5 mL | ||
| Colored membrane proteins | Ready-to-use buffers | PureCube Compact Cartridge, 5 mL |
| Bacteriorhodopsin | 8 M Urea | |
| Hs Sensory rhodopsin-1 | 0.5 M EDTA | Antibodies |
| Hs Sensory rhodopsin-2 | 10X PBS | PentaHis antibody |
| Np Sensory rhodopsin-2 | 50X TAE | GST antibody |
| Channelrhodopsin 1 | 10X TE | Rho1D4 antibody |
| 10X TBE | ||
| Green fluorescent proteins | 10X TBS | Eluent peptides |
| eGFP-His | Rho1D4 peptide | |
| GST-eGFP-Rho | ||
| GST-eGFP-DYKDDDDK |
StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"
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