Protocols & Datasheets

Cube Protocols & Datasheets

Our protocols for protein expression, purification and buffer optimization are constantly revised and updated. They are available for free download.
Protocols
Protein purification and detection protocols
CubicPhase Crystallization Protocol
General protocols
Nanodisc protocols
Cell-free protein expression protocol - Standard
Cell-free protein expression protocol - For T7 Lysates

Datasheets

General Protocols
Screening for optimal membrane protein expression conditions using E. coli expression systems
Screening detergents for optimal solubilization and purification of membrane proteins

Protocols for protein purification and detection

His affinity

For PureCube 100 INDIGO Agarose

PureCube 100 Ni-NTA Agarose

PureCube 100 Co-NTA Agarose,    PureCube Ni-NTA Agarose,

PureCube Co-NTA Agarose,

PureCube Ni-IDA Agarose

GST affinity Strep affinity Rho1D4  affinity Activated

Purification using

Affinity Agarose
 

native    denaturing

native native native

Amine

Carboxy

Epoxy

Maleimide

NHS

Thiol

Purification using 1-step Batch Midi Plus Columns

native    denaturing native native native

Carboxy

NHS

Purification using 1-step Batch Mini Columns native     denaturing native native native -
Purification using MagBeads native     denaturing native native native

Amine

Carboxy

Epoxy

Maleimide

NHS

Thiol

Regeneration

Cleaning and Regeneration

Cleaning and Regeneration Cleaning and Regeneration Cleaning and Regeneration -
Special applications
 
Nickel loading of IMAC Agarose
Al/Co/Cu/Fe/Zn loading of IMAC Agarose
Nickel loading of IMAC MagBeads
AL/Co/Cu/Fe/ZN loading of IMAC MagBeads
Pulldown using MagBeads
Western Blot detection Detection Detection - Detection -

Nanodisc protocols
  Membrane scaffold protein

MSP1D1

His-tagged

MSP1D1

MSP1D1-

deltaH5

His-tagged

MSP1D1-

deltaH5

MSP1E3D1

His-tagged

 

Reconstitution of detergent- solubilized membrane proteins using MSPs

UPDATED 01/16

DMPC

POPC

DMPC

POPC

DMPC

POPC

DMPC

POPC

DMPC

POPC

Nanodisc assembly for cell-free expression reactions - using Nanodisc Assembly Kits

UPDATED 02/16

DMPC

POPC

DMPC

POPC

DMPC

POPC

DMPC

POPC

DMPC

POPC


His Affinity Agaroses   His Affinity MagBeads   His Affinity Cartridges
Ni-IDA   Ni-IDA   Ni-IDA

PureCube Ni-IDA Agarose

 

PureCube Ni-IDA MagBeads

 

PureCube Ni-IDA Cartridge

PureCube Ni-IDA Agarose XL

      PureCube Compact Cartridge Ni-IDA
       
Ni-NTA   Ni-NTA   Ni-NTA

PureCube Ni-NTA Agarose

 

PureCube Ni-NTA MagBeads

 

PureCube Ni-NTA Cartridge

PureCube 100 Ni-NTA Agarose

      PureCube Compact Cartridge Ni-NTA

PureCube Ni-NTA Agarose XL

     

PureCube 100 Ni-NTA Cartridge

        PureCube 100 Compact Cartridge Ni-NTA
         
Co-NTA   Co-NTA   Co-NTA

PureCube Co-NTA Agarose

 

PureCube Co-NTA MagBeads

 

PureCube Co-NTA Cartridge

PureCube 100 Co-NTA Agarose

      PureCube Compact Cartridge Co-NTA

PureCube Co-NTA Agarose XL

     

PureCube 100 Co-NTA Cartridge

        PureCube 100 Compact Cartridge Co-NTA
         

INDIGO His affinity

 

INDIGO His affinity

 

INDIGO His affinity

PureCube 100 INDIGO Ni-Agarose

 

PureCube INDIGO Ni-MagBeads

 

PureCube 100 INDIGO Ni-Cartridge

       

PureCube 100 CompactCartridge Ni-INDIGO

         

Other NTA Matrices

 

Other NTA Matrices

   
Cu-NTA Agarose   Cu-NTA MagBeads    
Fe-NTA Agarose   Fe-NTA MagBeads    
Al-NTA Agarose   Al-NTA MagBeads    
Zn-NTA Agarose   Zn-NTA MagBeads    
Other Affinity Agaroses   Other Affinity MagBeads   Other Affinity Cartridges
Rho1D4   Rho1D4   Rho1D4
PureCube Rho1D4 Agarose   PureCube Rho1D4 MagBeads   PureCube Rho1D4 Cartridges
        PureCube Compact Cartridge Rho1D4
         
Glutathione   Glutathione   Glutathione

PureCube Glutathione Agarose

 

PureCube Glutathione MagBeads

 

PureCube Glutathione Cartridges

PureCube Glutathione Agarose XL

     

PureCube Compact Cartridge Glutathione

         
StrepTactin   StrepTactin   StrepTactin

PureCube HiCap StrepTactin Agarose

 

PureCube HiCap StrepTactin MagBeads

  PureCube HiCap StrepTactin Cartridges
        PureCube Compact Cartridge HiCap StrepTactin
Purified Proteins   Protein Crystallization   Accessories
Human GPCRs   CubeCrystal Plates   Columns to fill
GPBAR1/TGR5       PureCube 1-step batch Mini
OPRM1 in nanodisc       PureCube 1-step batch Midi Plus
GLP1R       PureCube Cartridge, 1 mL
NTSR1       PureCube Compact Cartridge, 1 mL
        PureCube Cartridge, 5 mL
Colored membrane proteins   Ready-to-use buffers   PureCube Compact Cartridge, 5 mL
Bacteriorhodopsin   8 M Urea    
Hs Sensory rhodopsin-1   0.5 M EDTA   Antibodies
Hs Sensory rhodopsin-2   10X PBS  

PentaHis antibody

Np Sensory rhodopsin-2   50X TAE   GST antibody
Channelrhodopsin 1   10X TE   Rho1D4 antibody
    10X TBE    
Green fluorescent proteins   10X TBS   Eluent peptides
eGFP-His      

Rho1D4 peptide

GST-eGFP-Rho        
GST-eGFP-DYKDDDDK        
StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"
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