Protocols & Datasheets

Cube Protocols & Datasheets

Our protocols for protein expression, purification and buffer optimization are constantly revised and updated. They are available for free download.

Cube Protocols

CubicPhase Crystallization Protocol
Cell-free protein expression protocol - Standard
Cell-free protein expression protocol - For T7 Lysates

General Protocols

Screening for optimal membrane protein expression conditions using E. coli expression systems

Screening detergents for optimal solubilization and purification of membrane proteins

Protocols for protein purification and detection

	All His affinity resins	GST affinity	Strep affinity	Rho1D4 affinity	Activated
Purification using Affinity Agarose	native denaturing	native	native	native	Amine Carboxy Epoxy Maleimide NHS Thiol
Purification using 1-step Batch Midi Plus Columns	native denaturing	native	native	native	Carboxy NHS
Purification using 1-step Batch Mini Columns	native denaturing	native	native	native	-
Purification using MagBeads	native denaturing	native	native	native	Amine Carboxy Epoxy Maleimide NHS Thiol
Regeneration	Cleaning and Regeneration	Cleaning and Regeneration	Cleaning and Regeneration	Cleaning and Regeneration	-
Special applications	Nickel loading of IMAC Agarose Al/Co/Cu/Fe/Zn loading of IMAC Agarose Nickel loading of IMAC MagBeads AL/Co/Cu/Fe/ZN loading of IMAC MagBeads	Pulldown using MagBeads	-	-	-
Western Blot detection	Detection	Detection	-	Detection	-

Nanodisc protocols

Membrane scaffold protein

	MSP1D1 His-tagged	MSP1D1	MSP1D1-deltaH5 His-tagged	MSP1D1-deltaH5	MSP1E3D1 His-tagged
Reconstitution of detergent- solubilized membrane proteins using MSPs UPDATED 01/16	DMPC POPC	DMPC POPC	DMPC POPC	DMPC POPC	DMPC POPC
Nanodisc assembly for cell-free expression reactions - using Nanodisc Assembly Kits UPDATED 02/16	DMPC POPC	DMPC POPC	DMPC POPC POPC	DMPC POPC	DMPC POPC

Cube Datasheets

His Affinity Agaroses	His Affinity MagBeads	His Affinity Cartridges
Ni-IDA	Ni-IDA	Ni-IDA
PureCube Ni-IDA Agarose	PureCube Ni-IDA MagBeads	PureCube Ni-IDA Cartridge
PureCube Ni-IDA Agarose XL		PureCube Compact Cartridge Ni-IDA
Ni-NTA	Ni-NTA	Ni-NTA
PureCube Ni-NTA Agarose	PureCube Ni-NTA MagBeads	PureCube Ni-NTA Cartridge
PureCube 100 Ni-NTA Agarose		PureCube Compact Cartridge Ni-NTA
PureCube Ni-NTA Agarose XL		PureCube 100 Ni-NTA Cartridge
		PureCube 100 Compact Cartridge Ni-NTA
Co-NTA	Co-NTA	Co-NTA
PureCube Co-NTA Agarose	PureCube Co-NTA MagBeads	PureCube Co-NTA Cartridge
PureCube 100 Co-NTA Agarose		PureCube Compact Cartridge Co-NTA
PureCube Co-NTA Agarose XL		PureCube 100 Co-NTA Cartridge
		PureCube 100 Compact Cartridge Co-NTA
INDIGO His affinity	INDIGO His affinity	INDIGO His affinity
PureCube 100 INDIGO Ni-Agarose	PureCube INDIGO Ni-MagBeads	PureCube 100 INDIGO Ni-Cartridge
		PureCube 100 CompactCartridge Ni-INDIGO
Other NTA Matrices	Other NTA Matrices
Cu-NTA Agarose	Cu-NTA MagBeads
Fe-NTA Agarose	Fe-NTA MagBeads
Al-NTA Agarose	Al-NTA MagBeads
Zn-NTA Agarose	Zn-NTA MagBeads

Other Affinity Agaroses	Other Affinity MagBeads	Other Affinity Cartridges
Rho1D4	Rho1D4	Rho1D4
PureCube Rho1D4 Agarose	PureCube Rho1D4 MagBeads	PureCube Rho1D4 Cartridges
		PureCube Compact Cartridge Rho1D4
Glutathione	Glutathione	Glutathione
PureCube Glutathione Agarose	PureCube Glutathione MagBeads	PureCube Glutathione Cartridges
PureCube Glutathione Agarose XL		PureCube Compact Cartridge Glutathione
StrepTactin	StrepTactin	StrepTactin
PureCube HiCap StrepTactin Agarose	PureCube HiCap StrepTactin MagBeads	PureCube HiCap StrepTactin Cartridges
		PureCube Compact Cartridge HiCap StrepTactin

Activated Agaroses	Activated MagBeads	Other Agaroses & MagBeads
		Unloaded IDA and NTA
PureCube Amine Agarose	PureCube Amine MagBeads	PureCube IDA Agarose
PureCube Carboxy Agarose	Pure Cube Carboxy MagBeads	PureCube IDA MagBeads
PureCube Epoxy Agarose	PureCube Epoxy MagBeads	PureCube NTA Agarose
PureCube Maleimide Agarose	PureCube Maleimide MagBeads	PureCube 100 NTA Agarose
PureCube NHS Agarose	PureCube NHS MagBeads	PureCube NTA MagBeads
PureCube Thiol Agarose	PureCube Thiol MagBeads
		Hydrophobic Interaction Matrices
		PureCube Butyl Agarose
		PureCube Octyl Agarose
		PureCube Phenyl Agarose
		Ion Exchange Matrices
		PureCube CM Agarose
		PureCube CM Agarose

Nanodisc scaffold proteins	Assembled nanodiscs	Nanodisc assembly kits
Human	Human	Human
MSP1D1 dH5-His	Nanodisc MSP1D1dH5-His_DMPC	MSP1D1 dH5-His_DMPC
MSP1D1 dH5	Nanodisc MSP1D1dH5-His_POPC	MSP1D1 dH5-His_POPC
MSP1D1-His	Nanodisc MSP1D1dH5-His_DMPG	MSP1D1-His_DMPC
MSP1D1	Nanodisc MSP1D1-His_DMPC	MSP1D1-His_POPC
MSP1E3D1-His	Nanodisc MSP1D1-His_POPC	MSP1E3D1-His_DMPC
MSP1E3D1	Nanodisc MSP1D1-His_DMPG	MSP1E3D1-His_POPC
MSP2N2-His	Nanodisc MSP1E3D1-His_DMPC
MSP2N2	Nanodisc MSP1E3D1-His_POPC
	Nanodisc MSP1E3D1-His_DMPG
Mouse/Rat	Nanodisc MSP2N2-His_DMPC
mouse MSP1D1-His	Nanodisc MSP2N2-His_POPC
mouse MSP1E3D1-His	Nanodisc MSP2N2-His_DMPG
rat MSP1D1-His	Nanodisc MSP1D1dH5-His_DMPC-BIotinyl-PE
rat MSP1E3D1-His	Nanodisc MSP1D1-His_DMPC-BIotinyl-PE
	Nanodisc MSP1E3D1-His_POPC-BIotinyl-PE
	Mouse/Rat
	Nanodisc mouse MSP1D1-His_DMPC
	Nanodisc mouse MSP1D1-His_POPC
	Nanodisc mouse MSP1E3D1-His_POPC
	Nanodisc mouse MSP1E3D1-His_POPC
	Nanodisc rat MSP1D1-His_DMPC
	Nanodisc rat MSP1D1-His_POPC
	Nanodisc rat MSP1E3D1-His_DMPC
	Nanodisc rat MSPE31D1-His_POPC

Purified Proteins	Protein Crystallization	Accessories
Human GPCRs	CubeCrystal Plates	Columns to fill
GPBAR1/TGR5		PureCube 1-step batch Mini
OPRM1 in nanodisc		PureCube 1-step batch Midi Plus
GLP1R		PureCube Cartridge, 1 mL
NTSR1		PureCube Compact Cartridge, 1 mL
		PureCube Cartridge, 5 mL
Colored membrane proteins	Ready-to-use buffers	PureCube Compact Cartridge, 5 mL
Bacteriorhodopsin	8 M Urea
Hs Sensory rhodopsin-1	0.5 M EDTA	Antibodies
Hs Sensory rhodopsin-2	10X PBS	PentaHis antibody
Np Sensory rhodopsin-2	50X TAE	GST antibody
Channelrhodopsin 1	10X TE	Rho1D4 antibody
	10X TBE
Green fluorescent proteins	10X TBS	Eluent peptides
eGFP-His		Rho1D4 peptide
GST-eGFP-Rho
GST-eGFP-DYKDDDDK

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"