Protocols & Datasheets
Cube Protocols & Datasheets
Our protocols for protein expression, purification and buffer optimization are constantly revised and updated. They are available for free download.
Protocols
Protein purification and detection protocols
CubicPhase Crystallization Protocol
General protocols
Nanodisc protocols
Cell-free protein expression protocol - Standard
Cell-free protein expression protocol - For T7 Lysates
Datasheets
Protein purification and detection protocols
CubicPhase Crystallization Protocol
General protocols
Nanodisc protocols
Cell-free protein expression protocol - Standard
Cell-free protein expression protocol - For T7 Lysates
Datasheets
General Protocols
Screening for optimal membrane protein expression conditions using E. coli expression systems
Screening detergents for optimal solubilization and purification of membrane proteins
Protocols for protein purification and detection
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His affinity For PureCube 100 INDIGO Agarose PureCube 100 Ni-NTA Agarose, PureCube 100 Co-NTA Agarose, PureCube Ni-NTA Agarose, PureCube Co-NTA Agarose, PureCube Ni-IDA Agarose |
GST affinity | Strep affinity | Rho1D4 affinity | Activated | |
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Purification using Affinity Agarose |
native | native | native | ||
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Purification using 1-step Batch Midi Plus Columns |
native denaturing | native | native | native | |
| Purification using 1-step Batch Mini Columns | native denaturing | native | native | native | - |
| Purification using MagBeads | native denaturing | native | native | native | |
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Regeneration |
Cleaning and Regeneration | Cleaning and Regeneration | Cleaning and Regeneration | - | |
| Special applications |
Nickel loading of IMAC AgaroseAl/Co/Cu/Fe/Zn loading of IMAC AgaroseNickel loading of IMAC MagBeadsAL/Co/Cu/Fe/ZN loading of IMAC MagBeads |
Pulldown using MagBeads | |||
| Western Blot detection | Detection | Detection | - | Detection | - |
Nanodisc protocols
| Membrane scaffold protein | ||||||||||||||
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MSP1D1 His-tagged |
MSP1D1 |
MSP1D1- deltaH5 His-tagged |
MSP1D1- deltaH5 |
MSP1E3D1 His-tagged |
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Reconstitution of detergent- solubilized membrane proteins using MSPs UPDATED 01/16 |
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Nanodisc assembly for cell-free expression reactions - using Nanodisc Assembly Kits UPDATED 02/16 |
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| His Affinity Agaroses | His Affinity MagBeads | His Affinity Cartridges | ||
| Ni-IDA | Ni-IDA | Ni-IDA | ||
| PureCube Compact Cartridge Ni-IDA |
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| Ni-NTA | Ni-NTA | Ni-NTA | ||
| PureCube Compact Cartridge Ni-NTA |
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| PureCube 100 Compact Cartridge Ni-NTA |
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| Co-NTA | Co-NTA | Co-NTA | ||
| PureCube Compact Cartridge Co-NTA |
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| PureCube 100 Compact Cartridge Co-NTA |
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INDIGO His affinity |
INDIGO His affinity |
INDIGO His affinity |
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Other NTA Matrices |
Other NTA Matrices |
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| Cu-NTA Agarose |
Cu-NTA MagBeads |
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| Fe-NTA Agarose |
Fe-NTA MagBeads |
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| Al-NTA Agarose |
Al-NTA MagBeads |
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| Zn-NTA Agarose |
Zn-NTA MagBeads |
| Other Affinity Agaroses | Other Affinity MagBeads | Other Affinity Cartridges | ||
| Rho1D4 | Rho1D4 | Rho1D4 | ||
| PureCube Rho1D4 Agarose |
PureCube Rho1D4 MagBeads |
PureCube Rho1D4 Cartridges |
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| PureCube Compact Cartridge Rho1D4 |
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| Glutathione | Glutathione | Glutathione | ||
| StrepTactin | StrepTactin | StrepTactin | ||
| PureCube HiCap StrepTactin Cartridges |
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| PureCube Compact Cartridge HiCap StrepTactin |
| Activated Agaroses | Activated MagBeads | Other Agaroses & MagBeads | ||
| Unloaded IDA and NTA | ||||
| PureCube Amine Agarose |
PureCube Amine MagBeads |
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| PureCube Carboxy Agarose |
Pure Cube Carboxy MagBeads |
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| PureCube Epoxy Agarose |
PureCube Epoxy MagBeads |
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| PureCube Maleimide Agarose |
PureCube Maleimide MagBeads |
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| PureCube NHS Agarose |
PureCube NHS MagBeads |
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| PureCube Thiol Agarose |
PureCube Thiol MagBeads |
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| Hydrophobic Interaction Matrices | ||||
| PureCube Butyl Agarose |
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| PureCube Octyl Agarose |
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| PureCube Phenyl Agarose |
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| Ion Exchange Matrices | ||||
| PureCube DEAE Agarose |
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| PureCube Q Agarose |
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| PureCube SP Agarose |
| Purified Proteins | Protein Crystallization | Accessories | ||
| Human GPCRs | CubeCrystal Plates |
Columns to fill | ||
| GPBAR1/TGR5 |
PureCube 1-step batch Mini |
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| OPRM1 in nanodisc |
PureCube 1-step batch Midi Plus |
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| GLP1R |
PureCube Cartridge, 1 mL |
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| NTSR1 |
PureCube Compact Cartridge, 1 mL |
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| PureCube Cartridge, 5 mL |
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| Colored membrane proteins | Ready-to-use buffers | PureCube Compact Cartridge, 5 mL |
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| Bacteriorhodopsin |
8 M Urea |
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| Hs Sensory rhodopsin-1 |
0.5 M EDTA |
Antibodies | ||
| Hs Sensory rhodopsin-2 |
10X PBS |
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| Np Sensory rhodopsin-2 |
50X TAE |
GST antibody |
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| Channelrhodopsin 1 |
10X TE |
Rho1D4 antibody |
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| 10X TBE |
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| Green fluorescent proteins | 10X TBS |
Eluent peptides | ||
| eGFP-His |
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| GST-eGFP-Rho |
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| GST-eGFP-DYKDDDDK |
StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"
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