Fe-NTA and GentleLys
are designed for proteomics experts to enhance enrichment strategies
Cube Biotech is a leading provider of innovative solutions for proteomics research and products enabling more reproducible results for targeted assays.
University of Washington
“I am a fan, so I definitely expect to use/test more of Cube Biotech GmbH products!”
Background:
Phosphoproteomics is a crucial field within proteomics research, studying the dynamics of protein phosphorylation in cell signaling pathways. However, achieving reliable and efficient enrichment of phosphorylated peptides can be challenging. Cube Biotech's Fe-NTA MagBeads offer a promising solution, Perkins states, as they provide “enhanced sensitivity, specificity, and deeper insights into phosphorylation dynamics through improved identification and quantification of phosphorylated peptides”.
Benefits and Features:
- Enhanced Sensitivity and Specificity: The Fe-NTA MagBeads enable researchers to achieve superior sensitivity and specificity in identifying and quantifying phosphorylated peptides, allowing for more accurate analysis of cell signaling events.
- Automation-Friendly: The phosphoproteomics procedure is simplified by the ease of automation of Fe-NTA MagBeads. The ability to incorporate these beads into automated processes reduces waiting time and boosts productivity. A great example from the Villén lab at UW led by Dr. Mario Leutert can be found here: https://pubmed.ncbi.nlm.nih.gov/31885202/.
- Superiority: In his 2019 publication Leutert et al. compared three different types of IMAC beads and TiO2 microspheres. Figure 1 highlights our PureCube Fe-NTA magnetic beads as the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 1 A, C) were enriched with the highest efficiency (Figure 1 B).
Fig.1: Comparison of phosphopeptide enrichment performance between four different products/methods. A: Number of unique phosphopeptides identified by the different enrichments (mean +/- SD, n = 3). B: Phosphopeptide enrichment efficiency shown as the fraction of phosphorylated peptides over total peptides (mean +/- SD, n = 3). C: Venn diagram of identified phosphopeptides by the different phosphopeptide enrichment methods. Source: Leutert et al. (2019) - Reusability: A notable advantage of Cube's Fe-NTA MagBeads is their reusability. By stripping the beads and reusing them several times, researchers can maximize cost-effectiveness while assuring quality control by evaluating the enrichment effectiveness after stripping.
- Cost-benefit Analysis: Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices (Fig. 2) for the frequently utilized phosphopeptide enrichment products (Fig. 1 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.
Fig.2: Cube Biotech does not only offer the best single product for phosphopeptide enrichment, but also the most prize efficient one. The products of Competitor G are ranging at about half the volume of beads you get for 200 USD in comparison to Cube Biotech. Suspension rates vary only slightly with 25% for the products of Cube Biotech, to 20% for Competitor G.
Application in Human Cell Lines and Tissues:
While initially optimized for yeasts, Cube Biotech's Fe-NTA MagBeads have proven to be “equally effective in human cell lines and tissues”. This versatility makes them a valuable tool for studying cell signaling and conducting phosphoproteomics analyses in various biological contexts.
Scientific Endorsement:
University of Washington researcher Thomas Perkins has used Cube Biotech's products frequently and especially suggests their Fe-NTA MagBeads for phosphoproteomic workflows. He has successfully employed these beads to enrich phosphorylated peptides from various cell lines, including stimulated HCT116s, HEK293Ts, HeLas, MCF7s, Hap1s, K562s, and many more.
GentleLys - Native Cell Lysis Buffer
Our GentleLys Buffer is the only 100% detergent-free, non-denaturing solution that allows lysing cultured cells from plate cultures as well as cell pellets from suspension cultures while solubilizing all membranes. It enables the extraction of soluble proteins and the stabilization of membrane proteins from all compartments of the cell. It is compatible with many applications, including Immunoassays, Protein Assays, Mass Spectrometry, and Protein Purification.
GentleLys - Native Cell Lysis Buffer
Our GentleLys Buffer is the only 100% detergent-free, non-denaturing solution that allows lysing cultured cells from plate cultures as well as cell pellets from suspension cultures while solubilizing all membranes. It enables the extraction of soluble proteins and the stabilization of membrane proteins from all compartments of the cell. It is compatible with many applications, including Immunoassays, Protein Assays, Mass Spectrometry, and Protein Purification.
Conclusion:
Cube Biotech's Fe-NTA MagBeads offer proteomics researchers an invaluable tool for optimizing phosphoproteomic workflows. With their enhanced sensitivity, automation-friendly design, reusability, and applicability to both yeasts and human cell lines, these beads enable more reproducible results and deeper insights into phosphorylation dynamics. Scientists, like Thomas Perkins, highly recommend Cube Biotech products, which also include nanodiscs, protein purification kits, and custom IMAC resins, making Cube Biotech GmbH a trusted partner for advanced proteomics research.
Keywords: Fe-NTA; MagBeads; Proteomics; Protein phosphorylation