Product information "PureCube Compact Cartridge Zn-NTA"
Type:
Cartridge
Bead size:
40 µm (Agarose)
Affinity :
His-tag, Zinc finger proteins
Purpose:
Protein Purification
Ligand:
NTA
Coupled Ion:
Zn2+
PureCube Zn-NTA COMPACT columns / cartridges were created for convenient FPLC based enrichment of zinc-ion binding proteins like zinc finger proteins. They can also be used for the purification of His tagges proteins. These columns / cartridges are pre-filled with our PureCube Zn-NTA agarose resin beads (40 µm in diameter). The PureCube COMPACT cartridge was developped for maximum compability with most commonly used FPLC hardware. It is still recommend to check the given features table . In case that no compability is given, we have another option. Please have a look at our other PureCube Zn-NTA cartridges / columns.
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Features
Usage
Specific binding and purification of 6x his-tagged proteins Enrichment of zinc finger proteins
Specifity
Affinity to His-tagged proteins Affinity to Zinc-ion binding proteins
Bead Ligand
Zn-NTA
Bead size
40 μm
pH stability/span>
2-14
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Filling quantity
Delivered as a 50 % suspension
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
8.0 x 35
17 x 35
Body material
Polypropylene
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
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