PureCube HiCap StrepTactin Agarose

Order number: 34101

€97.00*

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Description

Our PureCube StrepTactin® agarose resin beads consist of 6% cross-linked agarose that is coupled to the StrepTactin protein. They enable protein yields of up to 5 mg protein per ml resin.

They were developed for FPLC and batch spin-based protein purification. PureCube StrepTactin® agarose can also be purchased pre-packed in FPLC cartridges and columns Furthermore, PureCube StrepTactin® agarose XL resin is also available for higher FPLC flow rates or very viscous cell media. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads/MagBeads.
Feature
Usage Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
Specificity Affinity to Strep-tagged (WSHPQFEK) proteins
Binding capacity >5 mg/ml
Bead Ligand StrepTactin®
Bead size 100 μm
Matrix 6% cross-linked agarose
Recommended flow rates 0.25 - 1 ml/min
Max. flow rates 6 ml/min
Filling quantity Delivered as a 50 % suspension
Chemical stability 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
Short-term storage In neutral buffer at 4°C
Long-term storage In neutral buffer with 20% ethanol at 4°C
Required equipment
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer (incl. Rho1D4 peptide)
  • Ice bath
  • Refrigerated centrifuge for 50 ml tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

FAQ

Can I get the datasheet for the PureCube StrepTactin® Agarose?

Is StrepTactin® the same as Streptavidin?

No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.

Can I regenerate & reuse your StrepTactin® beads?

Disclaimer

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".