PureCube Compact Cartridge HiCap StrepTactin

Order number: 34302

€150.00*

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Description

Our HiCap StrepTactin® Compact Cartridge is a FPLC-ready column that is pre-packed with our PureCube HiCap StrepTactin® agarose resin.

Furthermore, PureCube StrepTactin® agarose resin is also available unpacked for alternative purification methods such as batch-spin and column chromatography. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads/MagBeads.

The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTATM.
Feature - StrepTactin® Agarose
Usage Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
Specificity Affinity to Strep-tagged (WSHPQFEK) proteins
Binding capacity >5 mg/ml
Bead Ligand StrepTactin®
Bead size 100 μm
Matrix 6% cross-linked agarose
Recommended flow rates 0.25 - 1 ml/min
Max. flow rates 6 ml/min
Filling quantity Delivered as a 50 % suspension
Chemical stability 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
Short-term storage In neutral buffer at 4°C
Long-term storage In neutral buffer with 20% ethanol at 4°C
Required equipment & materials
  • FPLC hardware like e.g. AEKTA
  • Binding Buffer
  • Elution Buffer


Feature - Column
PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Max. Flow Rate 6 mL/min 6 mL/min
Dimensions: diameter X length (mm) 5 X 35 17 X 35
Body material Polypropylene Polypropylene
Inlet 10-32 UNF female thread 10-32 UNF female thread
Outlet 10-32 UNF female thread 10-32 UNF female thread

FAQ

Can I get the datasheet for the PureCube HiCap StrepTactin® Compact Cartridge?

Is StrepTactin® the same as Streptavidin?

No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.

Can I regenerate & reuse your StrepTactin® beads?

Disclaimer

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".