PureCube Compact Cartridge HiCap StrepTactin

$154.80 *

Prices plus VAT plus shipping costs

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Delivery time appr. 1-3 workdays

mL:

  • 34302
PureCube HiCap StrepTactin COMPACT Cartridges / Columns are made with maximum compability... more
Product information "PureCube Compact Cartridge HiCap StrepTactin"
Type: Cartridge
Bead size: 100 µm (Agarose)
Affinity : Strep-tag
Purpose: Protein Purification


PureCube HiCap StrepTactin COMPACT Cartridges / Columns are made with maximum compability with the most common FPLC machinery in mind. In case that no compability between your FPLC hardware and these cartridges are given, please refer to our other StrepTactin Cartridges / Columns.
PureCube HiCap StrepTactin COMPACT cartridges are pre-filled with our high-quality PureCube HiCap StrepTactin Agarose for your convinience, because time spend in the lab to fill FPLC columns is better spend doing something else.

Features - Regarding Purification capabilities.

Usage Highly specific affinity purification of Strep® (WSHPQFEK) tagged proteins
Specifity Affinity to Strep® tagged proteins (WSHPQFEK)
Binding capacity 5 mg / ml Resin
Bead Ligand StrepTactin ®
Bead size 100 μm
Stability 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Features - Regarding Cartridge / Column itself.

Feature PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Dimensions: diameter X length (mm) 8.0 x 35 17 x 35
Body material Polypropylene
End Plug Material Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Max. Flow Rate 6 mL/min
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