SARS CoV-2 spike protein in DIBMA Glycerol

€986.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

µG:

  • 28706
SARS-CoV-2, more commonly known as the coronavirus, enters the cell by using its surface... more
Product information "SARS CoV-2 spike protein in DIBMA Glycerol"
Product Type: Spike protein
Protein Sequence: Full-length
SARS-CoV-2, more commonly known as the coronavirus, enters the cell by using its surface protein called SPIKE. SPIKE is processed on the cell's surface through the serine protease TMPRSS2 and subsequently binds to the surface receptor ACE2.
The native SPIKE protein is a trimer and is located in the coronavirus membrane. Therefore to obtain pure & native SPIKE the trimer needs to be kept intact. We achieved this in three different ways:
  1. MSP nanodiscs, based on MSP proteins
  2. Detergent Mycelles
  3. Synthetic nanodiscs, as you can see here
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in Synthetic Nanodisc, SDS page and Native Gel Figure 1: Data from our lab regarding SPIKE stabilized and purified in synthetic nanodiscs (Dibma Glycerol).
A: SDS of SPIKE in synthetic nanodisc.
B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl,
buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid)
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity Native Gel, see Fig. 1 C
Related links to "SARS CoV-2 spike protein in DIBMA Glycerol"
Spike Protein Delta Variant SARS CoV-2 full-length Spike B.1.617.2 (Delta)...
Article number: 28744
Sales price: From €258.00 *
SARS CoV-2 full-length Spike B.1.621 (Mu) Mutation SARS CoV-2 full-length Spike B.1.621 (Mu) Mutation
Article number: 28747
Sales price: From €258.00 *
B.1.429 Corona mutant available at Cube Biotech SARS CoV-2 full-length Spike B.1.429 Mutation
Article number: 28736
Sales price: From €258.00 *
SARS CoV-2 spike protein set in LMNG detergent (100 µg), DIBMA Glycerol (25 µg) and nanodisc complex SARS CoV-2 spike protein set in LMNG detergent...
Article number: 28704
Sales price: €2,654.00 *
SPIKE protein B.1.429 (CAl. 20C) SARS CoV-2 full-length Spike B.1.525 Mutation
Article number: 28733
Sales price: From €258.00 *
RB-Domain of the SARS-CoV 2 mutant strain P.1 SARS CoV-2 Spike P.1 Mutation RBD domain
Article number: 28731
Sales price: From €552.00 *
SARS CoV-2 Spike B.1.351 Mutation RBD domain SARS CoV-2 Spike B.1.351 Mutation RBD domain
Article number: 28729
Sales price: From €552.00 *
AChR T. Californica AChR T. Californica
Article number: 28601
Sales price: From €632.00 *
MC4R protein MC4R protein
Article number: 28401
Sales price: From €1,085.00 *
Tube Containing DIBMA Glycerol TRIS DIBMA Glycerol, TRIS
Article number: 18054
Sales price: From €229.00 *
PureCube Glutathione Cartridge PureCube Glutathione Cartridge
Article number: 32301
Sales price: From €47.00 *
OPRM1 protein nanodisc complex OPRM1 protein nanodisc complex
Article number: 29001
Sales price: From €1,838.00 *
Hs Bacteriorhodopsin Hs Bacteriorhodopsin
Article number: 28903
Sales price: €445.00 *
Tube Containing AASTY 11-55 AASTY 11-55
Article number: 18275
Sales price: From €209.00 *
Tube containing AASTY 6-55 AASTY 6-55
Article number: 18270
Sales price: From €209.00 *
Overview of all Synthetic polymers that are contained in the MAXI screening Kit Synthetic Nanodisc Screening Kit MAXI (16x2x50 mg)
Article number: 18295
Sales price: €650.00 *
AASTY 6-45 by Cube Biotech AASTY 6-45
Article number: 18250
Sales price: From €209.00 *
SARS-CoV-2 spike protein in SMALP 140 (25 µg) SARS-CoV-2 spike protein in SMALP 140 (25 µg)
Article number: 28711
Sales price: €1,282.00 *
SMALP Screening Set (4x3x50 mg) SMALP Screening Set (4x3x50 mg)
Article number: 18280
Sales price: €291.00 *
SMALP 140-I SMALP 140-I
Article number: 18230
Sales price: From €209.00 *
SMALP 140 SMALP 140
Article number: 18220
Sales price: From €209.00 *
SMALP 200 SMALP 200
Article number: 18210
Sales price: From €209.00 *
SMALP 300 SMALP 300
Article number: 18200
Sales price: From €209.00 *
Tube containing DIBMA Glucosamine TRIS DIBMA Glucosamine, TRIS
Article number: 18044
Sales price: From €229.00 *
Tube containing DIBMA Glucosamine DIBMA Glucosamine, HEPES
Article number: 18041
Sales price: From €229.00 *
Vial containing DIBMA 12 TRIS DIBMA 12, TRIS
Article number: 18014
Sales price: From €209.00 *
Vial containing DIBMA 12 HEPES DIBMA 12, HEPES
Article number: 18011
Sales price: From €209.00 *
Vial containing DIBMA 10 Tris DIBMA 10, TRIS
Article number: 18004
Sales price: From €209.00 *
Tube containing DIBMA 10 HEPES DIBMA 10, HEPES
Article number: 18001
Sales price: From €209.00 *
PureCube Compact Cartridge Fe-NTA PureCube Compact Cartridge Fe-NTA
Article number: 31602-Fe
Sales price: From €44.00 *
PureCube Al-NTA MagBeads PureCube Al-NTA MagBeads
Article number: 31501-Al
Sales price: From €80.00 *
10X Tris-EDTA (TE), pH 7.4 (10 L) 10X Tris-EDTA (TE), pH 7.4 (10 L)
Article number: 61278
Sales price: €242.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
Article number: 33101
Sales price: From €269.00 *
PureCube Glutathione Agarose PureCube Glutathione Agarose
Article number: 32103
Sales price: From €164.00 *
PureCube Compact Cartridge IDA PureCube Compact Cartridge IDA
Article number: 30902
Sales price: From €47.00 *
GST-eGFP fusion protein, DYKDDDDK-tag GST-eGFP fusion protein, DYKDDDDK-tag
Article number: 29921
Sales price: From €255.00 *
eGFP protein, His-tag eGFP protein, His-tag
Article number: 29901
Sales price: From €225.00 *
Np Sensory rhodopsin-2 Np Sensory rhodopsin-2
Article number: 28931
Sales price: €356.00 *
Hs Sensory rhodopsin-2 Hs Sensory rhodopsin-2
Article number: 28921
Sales price: €356.00 *
NTSR1 protein NTSR1 protein
Article number: 28301
Sales price: From €1,085.00 *
Viewed