HighSpec Rho1D4 Agarose
Order number: 33103
Description
HighSpec Rho1D4 Agarose resin is a suspension of 40 µm agarose beads, coupled to an anti-Rho1D4 antibody.
It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. More on the Rho1D4-tag can be read here.
You can also purchase these agarose beads pre-packed into FPLC-ready cartridges. Additionally, we offer Rho1D4-tag affinity resin with a bead diameter of 400 µm for very viscous cell media and higher FPLC flow rates. Alternatively, Rho1D4 MagBeads are also available, if you prefer this affinity chromatography method.
Additional support products for Rho1D4-tag purification assays are Rho1D4 peptide, an essential compound of the elution buffer, and our Rho1D4 antibody for later western blot detection of your protein.
You are using the Rho1D4-tag for the first time? For this case, we recommend using one of our three Rho1D4 Starter Kits. Each combined one of our Rho1D4-tag purification products + 5 mg of the Rho1D4 elution peptide.
You can also purchase these agarose beads pre-packed into FPLC-ready cartridges. Additionally, we offer Rho1D4-tag affinity resin with a bead diameter of 400 µm for very viscous cell media and higher FPLC flow rates. Alternatively, Rho1D4 MagBeads are also available, if you prefer this affinity chromatography method.
Additional support products for Rho1D4-tag purification assays are Rho1D4 peptide, an essential compound of the elution buffer, and our Rho1D4 antibody for later western blot detection of your protein.
You are using the Rho1D4-tag for the first time? For this case, we recommend using one of our three Rho1D4 Starter Kits. Each combined one of our Rho1D4-tag purification products + 5 mg of the Rho1D4 elution peptide.
Datasheets
Feature | |
---|---|
Usage | Specific binding and purification of Rho1D4-tagged proteins |
Specificity | Affinity to Rho1D4-tagged (TETSQVAPA) proteins |
Binding capacity | >3 mg/ml |
Bead Ligand | anti-Rho1D4 antibody |
Bead size | 100 μm |
Matrix | 6% cross-linked agarose |
Recommended flow rates | 0.25 - 1 ml/min |
Max. flow rates | 6 ml/min |
Filling quantity | Delivered as a 50 % suspension |
Short-term storage | In neutral buffer at 4°C |
Long-term storage | In neutral buffer with 20% ethanol at 4°C |
Required equipment |
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Citations
Lab Results
Rho1D4-tag purification products are especially handy when it comes to the purification of proteins with low abundance. And a huge group of these proteins is membrane proteins. Even overexpressed their concentration does not come close to their soluble counterparts.
Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
FAQ
Can I get the datasheet for the Highspec Rho1D4 Agarose?
Can I regenerate & reuse your Glutathione beads?
Can I use the Rho1D4-tag also for non-membrane proteins?
Yes, you can.
I never heard about the Rho1D4-tag before. What can you tell me about it?
Do not worry. All your questions should be answered on our Rho1D4-tag knowledge page.
I use the FLAG-tag (DYKDDDDK) for my protein purifications. Is it beneficial for me to switch to the Rho1D4-tag?
Yes, it definetly is! Have a look at this side-by-side comparison of the two.