Alprenolol

Purifying GPCRs on a ligand-based affinity column can yield an active, homogeneous preparation. Whenever a specific ligand for a GPCR of interest is known, we can attempt to couple it to one of our activated agaroses or magnetic beads to yield a purification matrix that is specific to the active conformation of this protein. Alprenolol agarose is a good example of ligand-based purification. It binds to the beta 1 and beta 2 adrenergic receptors and the ligand was coupled via a hydrophilic linker to our highly crosslinked 6% agarose to minimize non-specific binding. This matrix is available off the shelf in mL quantities. Please contact us for alprenolol magbeads, or for bulk quantities of this matrix.