Purification of GPCRs via affinity tags can yield highly pure proteins, but due to the high flexibility of the proteins typically only a fraction of the entire preparation is active. This is often reflected in SEC spectra that show different stages of aggregation. Purifying the same GPCR on a ligand-based affinity column can yield an active, homogeneous preparation. Whenever a specific ligand for a GPCR of interest is known, we can attempt to couple it to one of our activated agaroses or magnetic beads to yield a purification matrix that is specific to the active conformation of this protein. One well-known example for such a ligand-based purification is Alprenolol Agarose, which binds to the beta 1 and beta 2 adrenergic receptors. The ligand was coupled via a hydrophilic linker to our highly crosslinked 6% agarose to minimize non-specific binding. This matrix is available off the shelf in mL quantities. Please
contact us for alprenolol magbeads, or for bulk quantities of this matrix.
Alprenolol-affinity products from Cube Biotech were successfully used in the following publications:
| Protein | Year | Author | Used Product |
|---|
| Native β1 Adrenergic Receptor | 2019 | Sun R., Mak S., Haschemi J., Horn P., Boege F., Luppa P.B.,1 | Alprenolol Agarose |
| RIG-I-like receptor LGP2 | 2019 | van der Veen A., Maillard P. V., Schmidt J.M., Lee S.A., Deddouche S., Borg A., Kjær S., Snijders B., e Sousa C.R., 2 | Alprenolol MagBeads |
Features
| Usage | Specific binding to ß-adrenergic receptors and alkene functions |
| Specifity | Affinity to ß-adrenergic receptors and alkene functions |
| Filling quantity | Delivered as a 50 % suspension |
| Bead size | 100 μm |
| Storage | Long term storage in 100 mM sodium hydrogencarbonate, 0.02% sodium azide, pH 7.5, ethanol at 4 °C. |
| Bead Ligand | Alprenolol |
References
- Sun, R: et al. (2019). Nanodiscs Incorporating Native β1 Adrenergic Receptor as a Novel Approach for the Detection of Pathological Autoantibodies in Patients with Dilated Cardiomyopathy. The Journal of Applied Laboratory Medicine. jalm.2018.028225. 10.1373/jalm.2018.028225.
- Van der Veen, A.G. et al. “The RIG-I-like receptor LGP2 inhibits Dicer-dependent processing of long double-stranded RNA and blocks RNA interference in mammalian cells.” The EMBO journal vol. 37,4 (2018): e97479. doi:10.15252/embj.201797479
