Purification of GPCRs via affinity tags can yield highly pure proteins, but due to the high flexibility of the proteins typically only a fraction of the entire preparation is active. This is often reflected in SEC spectra that show different stages of aggregation. Purifying the same GPCR on a ligand-based affinity column can yield an active, homogeneous preparation. Whenever a specific ligand for a GPCR of interest is known, we can attempt to couple it to one of our activated agaroses or magnetic beads to yield a purification matrix that is specific to the active conformation of this protein. One well-known example for such a ligand-based purification is Alprenolol Agarose, which binds to the beta 1 and beta 2 adrenergic receptors. The ligand was coupled via a hydrophilic linker to our highly crosslinked 7.5% agarose to minimize non-specific binding. This matrix is available off the shelf in mL quantities. Please contact us for alprenolol magbeads, or for bulk quantities of this matrix.

Alprenolol-affinity products from Cube Biotech were successfully used in the following publications:

 ProteinYearAuthorUsed Product
Native β1 Adrenergic Receptor 2019  Sun R., Mak S., Haschemi J., Horn P., Boege F., Luppa P.B.,1 Alprenolol Agarose
RIG-I-like receptor LGP2 2019  van der Veen A., Maillard P. V., Schmidt J.M., Lee S.A., Deddouche S., Borg A., Kjær S., Snijders B., e Sousa C.R., 2 Alprenolol MagBeads


Usage Specific binding to ß-adrenergic receptors and alkene functions
Specifity Affinity to ß-adrenergic receptors and alkene functions
Filling quantity Delivered as a 50 % suspension
Bead size 40 μm
Storage Long term storage in 100 mM sodium hydrogencarbonate, 0.02% sodium azide, pH 7.5, ethanol at 4 °C.
Bead Ligand Alprenolol


  1. Sun, R: et al. (2019). Nanodiscs Incorporating Native β1 Adrenergic Receptor as a Novel Approach for the Detection of Pathological Autoantibodies in Patients with Dilated Cardiomyopathy. The Journal of Applied Laboratory Medicine. jalm.2018.028225. 10.1373/jalm.2018.028225.
  2. Van der Veen, A.G. et al. “The RIG-I-like receptor LGP2 inhibits Dicer-dependent processing of long double-stranded RNA and blocks RNA interference in mammalian cells.” The EMBO journal vol. 37,4 (2018): e97479. doi:10.15252/embj.201797479
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PureCube Alprenolol Agarose PureCube Alprenolol Agarose
Article number: 56031
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PureCube Alprenolol Agarose PureCube Alprenolol Agarose
100 % specialized for protein purification of ß-adrenergic receptor proteins. Alprenolol Agarose resin for batch spin & FPLC.
Article number: 56031
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