L-Glutathione reduced

Order number: 61035

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Description

Reduced L-Glutathione is the natural ligand of the protein Glutathione-S-Transferase or GST. It is, therefore, an essential component for GST-tag-based protein purification assays.
Loose L-Glutathione (reduced) is a compound of the elution buffer of GST-tag purification assays. It works by giving the GST-tag an alternative substrate to bind to instead of the L-Glutathione on the involved purification beads.
ParameterMethodSpecificationResult
Appearance   White powder Corresponds
Amount per Bottle   25 g  
Usage   Competetive elution of GST tagged proteins from GST affinity beads  
Assay (HPLC)   Min. 97% 99.6%
Spec. rotation ([α]20 D) : 2% in water -16° - -19° -16.3°
Soluble in: 5% in water Clear, colourless solution Corresponds
Solubility   Approx. 100 g/l (H2O) Corresponds
Heavy Metals (as Pb)   Max. 0.0005% Corresponds
Formula   C10H17N3O6S  
Molecular Weight   307.33 g/mol  
Cas #   70-18-8  

Citations

Purified ProteinYearAuthor
Several TBX3 fusion proteins 2015 Fischer K.
RDP215 variants 2023 Pasupuleti R., Riedl S., Núñez L.S., Karava M., Kumar V., Kourist R., Turnbull W.B., Zweytick D., Wiltschi B.