For maximum purity of His-tagged protein Co-NTA is the best metal-ion + ligand option. As magnetic beads the purification process is easy to perform and does not require space-intensive tools. In case bigger MagBeads are desired please consider our For our Co-NTA MagBeads XL (~90 µm). Of course we are also offering our own PureCube MagBead separator for the separation process.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x His-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Bead Ligand
Co-NTA
Bead size
30 µm
Filling quantity
Delivered as a 25 % suspension
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
