PureCube XL Co-NTA agarose consist of agarose beads with a diameter of around 400 µm. They are used for extreme cases of His tag protein purifications. These extremes cases are defined by very low flow rates. XL beads can be used for FPLC assays to overcome this issue. For Co-NTA based His Tag purification we recommend our PureCube Co-NTA resin with 40 µm diameter and our Purecube 100 Co-NTA agarose resin beads.
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Features
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity
Delivered as a 50 % suspension
Bead size
400 μm
Bead Ligand
Co-NTA
Required equipment
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
50 mL centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
