Purification Resins
Ni-IDA is a abbreviation for a nickel ion that has been coupled to Iminodiacetic acid (IDA). In the field of protein purification it is used for His tag purifications. It can be used either as a agarose resin for FPLC or as magnetic beads. Cube Biotech offers high quality Ni-IDA products for your IMAC assays.

Overview of Cube Biotech's Ni-IDA products for His tag protein purification.


Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >70 mg/mL
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Bead Ligand Ni-IDA

Ni-IDA products from Cube Biotech was successfully used in the following publications:

UGDH, NOX, GalK, USP, GalT and GlcAT 2019 Heinzler R., Fischöder T., Elling L., Franzreb M.1 Ni-IDA agarose 40 µm

Our Ni-IDA Resins in detail

Cube Biotech Ni-NTA compared to competitor G
Fig. 1: The binding capacity of PureCube Ni-IDA Agarose is comparable to resins from a leading supplier. SDS-PAGE of CAT purified in gravity columns with PureCube Ni-IDA Agarose and Ni resin from Competitor G show elution fractions (E1-E5) with similar protein yields.
Strong and robust performance
Based on the same agarose matrix with high porosity and physical stability, PureCube Ni-IDA agarose exhibits a protein capacity of 50 mg protein per mL resin, which is competitive with that of products from market-leading providers. Figure 1 shows the SDS-PAGE analysis of 6-His chloramphenicoltransferase (CAT) expressed in E. coli and purified on gravity flow columns filled with PureCUbe Ni-IDA Agarose or Competitor G Ni-Sepharose High Performance resin. The amount of protein drawn down from the columns in 5 elution fractions was comparable for the two affinity resins (CL: cleared lysate; FT: flow-through; W1-2: wash fractions; E1-5: elution fractions). Table 1 compares additional parameters among PureCube Ni-IDA Agarose and two equivalent market-leading resins.
Top-quality materials, competitive performance
PureCube Ni-IDA Agarose is produced with a highly cross-linked agarose which is physically very stable and suitable for purification processes under low pressure (bulk purifications as well as packed in chromatography columns). Additionally, the high porosity and uniform size of the matrix allows for optimal protein interaction and high reproducibility between individual purification runs. Table 1 summarizes technical and performance parameters of PureCube Ni-IDA Agarose and 2 equivalent, market-leading resins.
Table 1. Comparision of three Ni-IDA manufacturers on the market.
  Particle SizeMetal ion capacityBinding capacitypH stabilityRecommended flow rateDTT stabilityEDTA stability
Cube Biotech 32-60 µm >25 µmol/mL/mL 70 mg/mL 3.0-13.0 0.5-2.0mL/min (6.0 mL/min possible) 10 mM 1.0 mM
Competitor G average 90 µm >30 µmol/mL 15 mg/mL 3.0-13.0 1.0 mL/min No information No information
Competitor S 45-160 µm 6-18 µmol/mL 70 mg/mL Not provided 1.0 mL/min 5 mM Not recommended


  1. Heinzler, R. et al. (2019). Toward Automated Enzymatic Glycan Synthesis in a Compartmented Flow Microreactor System. Advanced Synthesis & Catalysis. 10.1002/adsc.201900709.
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PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
2 ml 50% IDA Agarose, loaded with nickel (II) sulfate
Article number: 30103
Sales price: From €70.00 *
PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
1 ml 25% IDA magnetic beads, loaded with nickel (II) sulfate
Article number: 30201
Sales price: From €43.00 *
PureCube Ni-IDA Cartridge PureCube Ni-IDA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30301
Sales price: From €33.00 *
PureCube Compact Cartridge Ni-IDA PureCube Compact Cartridge Ni-IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30302
Sales price: From €33.00 *
PentaHis antibody, BSA-free (0.1 mg) PentaHis antibody, BSA-free (0.1 mg)
Lyophilized. Manufactured by QIAGEN.  
Article number: 40040
Sales price: €448.00 *
PureCube Ni-IDA Agarose XL PureCube Ni-IDA Agarose XL
2 ml 50% Ni-IDA Agarose with medium particle size of 400 micrometer, for special applications
Article number: 55003
Sales price: From €101.00 *
PureCube Ni-IDA MagBeads XL PureCube Ni-IDA MagBeads XL
1 ml 25% IDA magnetic beads, loaded with nickel (II) sulfate. Particle size 70-120 micrometer, for special applications.
Article number: 55205
Sales price: From €222.00 *