Ni-NTA
The term Ni-NTA (Nickel NTA) refers to a nickel2+ ion that has been coupled to Nitrilotriacetic acid (NTA). Ni-NTA can then be coupled to agarose resin or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. The size of the used agarose resin beads or magnetic beads influences the flow rates and the protein yield.
