PureCube 100 Ni-NTA Agarose

PureCube 100 Ni-NTA Agarose
€112.00 *

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ML:

  • 74103
Our PureCube 100 Ni-NTA agarose resins are beads with a diameter of 100 µm. They are used... more
Product information "PureCube 100 Ni-NTA Agarose"
Type: Agarose
Bead size: 100 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: NTA
Coupled Ion: Ni2+
Our PureCube 100 Ni-NTA agarose resins are beads with a diameter of 100 µm. They are used for the purification of active His tagged proteins from cells. Years of experience in manufacturing and developing agarose beads have accumulated to the high protein yield of 80 mg protein per ml resin, which is leading in the market compared to other Ni-NTA suppliers. PureCube 100 Ni-NTA resins are suited for FPLC and batch spin columns. For more mechanical stable agarose beads we recommend our Ni-NTA agarose beads with 40 µm diameter and if an even higher flow rate is desired our XL sized Ni-NTA beads.

Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Bead size 100 μm
Binding capacity >80 mg/mL
Bead Ligand Ni-NTA
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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