PureCube Cu-NTA Agarose
Order number: 31403-Cu
Description
Our PureCube Cu-NTA agarose resins are small agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells and the enrichment of Copper-binding proteins. Our year-long experience in manufacturing agarose resin lead to the high yield of 80 mg protein per ml resin, which is leading in the market compared to other Cu-NTA suppliers. PureCube Cu-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with great mechanical stability.
Furthermore, you can also get these Cu-NTA beads pre-packed in a column/cartridge or as magnetic beads.
Furthermore, you can also get these Cu-NTA beads pre-packed in a column/cartridge or as magnetic beads.
Datasheets
- Cu-NTA agarose Datasheet
- Native Purification Protocol
- Denaturing Purification Protocol
- Batchspin MINI Native Purification Protocol
- Batchspin MINI Denaturing Purification Protocol
- Batchspin MIDI Native Purification Protocol
- Batchspin MIDI Denaturing Purification Protocol
- Washing & Regeneration Protocol
- Cartridge Packing Protocol
Feature | |
---|---|
Usage | Specific binding and purification of 6x His-tagged proteins Binding of Copper-binding proteins |
Specificity | Affinity to His-tagged proteins Affinity to Copper-binding proteins |
Binding capacity | >80 mg/mL |
Bead Ligand | Cu-NTA |
Bead size | 40 μm |
Filling quantity | Delivered as a 50 % suspension |
Metal ion capacity | > 15 µeqv Cu2+/ml |
pH stability | 2-14 |
Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
Required equipment |
|
Citations
Purified Protein | Year | Author |
---|---|---|
HrpII | 2017 | Bauer W.S., Richardson K.A., Adams N.M., Ricks K.M., Gasperino D.J., Ghionea S.J., Rosen M., Nichols K.P., Weigl B.H., Haselton F.R., Wright D.W. |
Lab Results
Different metal ions confer different binding affinity and specificity
Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, copper exhibits the highest protein yield of commonly used IMAC metal ions, leading to relatively lower purity. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, copper exhibits the highest protein yield of commonly used IMAC metal ions, leading to relatively lower purity. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Video
Video Guide - How to pack FPLC cartridges
Video Guide - FPLC
Video Guide - Column Chromatography
Video Guide - Batch Spin Chromatography
FAQ
Can I get the datasheet for the Cu-NTA resin?
What are the reasons for non-specific binding?
Some protein can bind to Cu-NTA even without having a His-tag. But to a lesser extent. Washing with NaOH after elution of your protein of interest removes unspecific bound proteins from your resin.
I want to use a high concentration of EDTA and DTT. Is it possible to use Zn-NTA from Cube Biotech?
No, it is not recommended because nickel-ions are reduced with DTT or dissolved with EDTA. If you want to use high concentrations of EDTA and DTT you should use our Indigo resin.
Can Cu-NTA beads be stripped?
Yes, they can. However, we recommend using our pre-stripped NTA or IDA beads, to begin with.
After using DTT my resin changed color. How to regenerate it?
The DTT has probably destroyed your beads. Cu-NTA beads only have a very limited DTT tolerance. However, you can regenerate them to regain their functionality. Please read our detailed protocol for more information regarding this. It is linked above.
However, we would recommend using Ni-INDIGO products instead. They work with the same buffers and protocols as the Cu-NTA products but have a DTT tolerance of 20 mM.
However, we would recommend using Ni-INDIGO products instead. They work with the same buffers and protocols as the Cu-NTA products but have a DTT tolerance of 20 mM.