Our PureCube Fe-NTA agarose resin beads were developed for the purification of phosphorylated peptides. A second possible use of the Fe-NTA MagBeads is purification of proteins that carry a poly-his tag. The affinity matrix is based on 7.5 % cross-linked agaros. The average bead size is 40 µm with a great homogenicity, that results in great reproducibility. . Alternatively Cube Biotech also offers PureCube Fe-NTA Magnetic Beads / MagBeads. Furthermore this Fe-NTA resin can be obtained pre-packed in PureCube cartridges/columns.
Features - Regarding Purification capabilities.
Feature
Usage
Enrichment of phosphopeptides
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Affinity to phosphorylated biomolecules
Matrix
7.5% cross-linked agarose
Bead Ligand
Fe-NTA
Bead size
40 µm
Filling quantity
Delivered as a 50 % suspension
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
