PureCube Fe-NTA Agarose
Order number: 31403-Fe
Description
PureCube Fe-NTA agarose resin beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of his tagged proteins as a secondary function. The affinity matrix is based on 7.5% cross-linked agarose that was magnetized. The bead ligand is Fe-NTA and the particle diameter is 40 µm. They are very homogeneous in size, yielding in a high degree of reproducibility.
As an alternative we also offer PureCube Fe-NTA MagBeads and this resin pre-packed in cartridges/columns.
As an alternative we also offer PureCube Fe-NTA MagBeads and this resin pre-packed in cartridges/columns.
Datasheets
- Fe-NTA Agarose Datasheet
- Phosphopeptide Enrichment Protocol
This protocol is written for the use of magnetic beads use it as a reference to create a corresponding agarose resin protocol.
Feature | |
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Usage |
|
Specificity |
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Bead ligand | Fe-NTA |
Bead size | 40 µm |
Filling quantity | Delivered as a 50 % suspension |
Matrix | 7.5% crosslinked agarose |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
pH stability | 2-14 |
Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
Citations
Lab Results
Automatic Phosphoproteomics for high-throughput projects
Fe-NTA magnetic beads by Cube Biotech have been proven to increase the speed of a high throughput experiment drastically. Leutert et al. (2019) presented the application of PureCube Fe-NTA MagBeads in a procedure that they named R2-P2, which is short for Rapid-Robotic PhosphoProteomics. They used a KingFisherTM Flex for their robotic runs to fully automize the phosphopeptide enrichment process.
Fe-NTA magnetic beads by Cube Biotech have been proven to increase the speed of a high throughput experiment drastically. Leutert et al. (2019) presented the application of PureCube Fe-NTA MagBeads in a procedure that they named R2-P2, which is short for Rapid-Robotic PhosphoProteomics. They used a KingFisherTM Flex for their robotic runs to fully automize the phosphopeptide enrichment process.
Superiority over other phosphopeptide enrichment methods
Leutert et al. compared three different types of IMAC beads (including our PureCube Fe-NTA) and TiO2 microspheres. As it can be seen in figure 2 our PureCube Fe-NTA magnetic beads presented themselves to be the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 2 A and C) were enriched with the highest efficiency (Figure 2 B).
Leutert et al. compared three different types of IMAC beads (including our PureCube Fe-NTA) and TiO2 microspheres. As it can be seen in figure 2 our PureCube Fe-NTA magnetic beads presented themselves to be the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 2 A and C) were enriched with the highest efficiency (Figure 2 B).
The best product for the best prize
Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices for the frequently utilized phosphopeptide enrichment products (Fig. 2 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.
Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices for the frequently utilized phosphopeptide enrichment products (Fig. 2 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.
FAQ
Can I get the datasheet for the Fe-NTA MagBeads?
What can I do with Fe-NTA beads?
Fe-NTA beads serve two purposes. First, they can be used as the matrix to bind and thus enrich phophopeptides. Second, they can be used similar to e.g Ni-NTA to purify His-tagged proteins via IMAC.
How does the phosphopeptide enrichment process work?
Use this protocol as a reference. It is written for our PureCube Fe-NTA MagBeads, so you have to make some adjustments to it.
Can I enrich all phosphopeptides using Fe-NTA?
No, this is not possible. Not all phosphopeptides bind to Fe-NTA beads. As shown in figure 2 multiples bead types must be used to cover all phosphopeptides off a cell. Because of that we also offer Ti- Zr- and Al-NTA beads.