PureCube Rho1D4 Agarose

PureCube Rho1D4 Agarose
€239.00 *

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ML:

  • 33101
Rho1D4 refers to a nine amino acid long affinity tag (TETSQVAPA), which can be used for... more
Product information "PureCube Rho1D4 Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : Rho1D4-tag
Purpose: Protein Purification
Rho1D4 refers to a nine amino acid long affinity tag (TETSQVAPA), which can be used for highly specific affinity protein purification. It even outshines the more commonly used Flag-Tag in both protein yield and purity. Our PureCube Rho1D4 agarose resin was made for FPLC protein purification assays. It is composed of 40 µm agarose beads that are functionalized through the Rho1D4 antibody. This product is also availible as magnetic beads or XL Agarose resins for higher flow rates.
First time working with Rho1D4? Try out our Rho1D4 Starting Set 1. It combines PureCube Rho1D4 Agarose and the Rho1D4 protein elution peptide.
rho1d4 tag membrane protein Schematic of a membrane protein tagged with Rho1D4

Features

 
Usage Highly specific affinity purification of Rho1D4 (TETSQVAPA) tagged proteins
Specifity Affinity to Rho1D4 tagged proteins
Special feature Highly recommended for the purification of membrane proteins.
Binding capacity 3-4 mg / ml Resin
Bead Ligand Rho1D4 Antibody
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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