Rho Starter Set 1: HighSpec Rho1D4 Agarose (1 ml) + HighSpec Rho1D4 Peptide (5 mg)

Order number: 33199

€414.00*

€228.00*

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Description

Our Rho1D4 Starter Set 1 is a combination of our PureCube Rho1D4 Agarose resin and the Rho1D4 elution peptide. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. More on the Rho1D4-tag can be read here.

Do you prefer magnetic beads over agarose resin or would like to have the Rho1D4 agarose pre-packed into an FPLC column? No problem, as we also offer other Rho1D4 Starter Sets.

Furthermore, we recommend our Rho1D4 antibody for later western blot detection of your protein.

Datasheets

Feature - Rho1D4 Agarose
Usage	Specific binding and purification of Rho1D4-tagged proteins
Specificity	Affinity to Rho1D4-tagged (TETSQVAPA) proteins
Binding capacity	>3 mg/ml
Bead Ligand	anti-Rho1D4 antibody
Bead size	100 μm
Matrix	6% cross-linked agarose
Recommended flow rates	0.25 - 1 ml/min
Max. flow rates	6 ml/min
Filling quantity	Delivered as a 50 % suspension
Short-term storage	In neutral buffer at 4°C
Long-term storage	In neutral buffer with 20% ethanol at 4°C
Required equipment	Lysis Buffer Wash Buffer Elution Buffer (incl. Rho1D4 peptide) Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Feature - Rho1D4 Elution Peptide
Usage	Elution of Rho1D4-tagged protein from matching purification beads
Specifity	Affinity to Rho1D4 affinity beads
Sequence	Ac_TETSQVAPA_NH₂
Amount	5 mg per tube
Storage, short term	Store lyophilized and reconstituted peptide at 4°C
Storage, long term	Store lyophilized and reconstituted peptide at-20°C

Citations

Purified Protein	Year	Author
Rhodopsin Mutants	2015	Mattle D., Singhal A., Schmid G., Dawson R., Standfuss J.
PRG2	2019	Brosig A., Fuchs J., Ipek F., Kroon C., Schrötter S., Vadhvani M., Polyzou A., Ledderose J., van Diepen M., Holzhütter H-G., Trimbuch T., Gimber N., Schmoranzer J., Lieberam I., Rosenmund C., Spahn C., Scheerer P., Szczepek M., Leondaritis G., Eickholt B.J.
Opsin	2019	Napolitano A., van der Veen A.G., Bunyan M., Borg A., Frith D., Howell S., kjaer S., Beling A., Snijders A.P., Knobeloch K-P., Frickel E-M.
NeoR	2020	Broser M., Spreen A., Konold P.E., Peter E., Adam S., Borin V., Schapiro I., Seifert R., Kennis J.T.M., Sierra Y.A.B., Hegemann P.
Gαs	2021	Swonger Koretz K., McGraw C.E., Stradley S., Elbaradei A., Malstadt N., Robinson A.S.
emST	2021	Bradberry M.M., Mishra S., Zhang Z., Wu L., McKetney J.M., Vestling M.M., Coon J.J., Chapman E.R
Immunoisolation of synpatic vesicles	2022	Weihou G.
	2022	Rozenberg A., Kaczmarczyk I., Matziv D., Verock J., Nagata T., Sugiura M., Katayama K., Kawasaki Y., Konno M., Nagasaka Y., Aoyama M., Das I., Pahima E., Church J., Adam S., Borin V.A., Chazan A., Augustin S., Wietek J., Diene J., Peleg Y., Kawanabe A., Fujiwara Y., Yizhar O., Sheves M., Schapiro I., Furutani Y., Hideki K., Keeichi I., Hegemann P., Béjà O., Shalev-Benami

Lab Results

Rho1D4-tag purification products are especially handy when it comes to the purification of proteins with low abundance. And a huge group of these proteins is membrane proteins. Even overexpressed their concentration does not come close to their soluble counterparts.

Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.

Examples for RHo1D4-tag protein purifications using Cube Biotech products — **Figure 1:** Example of Rho1D4-tag purifications using Rho1D4 MagBeads. The four depicted anti-Rho1D4 western blots show one different mutant of the SARS-CoV-2 surface protein SPIKE. A: Alpha mutant B.1.1.7 B: Beta mutant B.1.351 C: Epsilon mutant B.1.429 D: Eta mutant B.1.525

FAQ

Can I get the datasheet for the PureCube Rho1D4 Agarose or Rho1D4 peptide?

Yes, Just click to download both here.
Rho1D4 Agarose Datasheet
Rho1D4 Peptide Datasheet

Can I use the Rho1D4-tag also for non-membrane proteins?

Yes, you can.

I never heard about the Rho1D4-tag before. What can you tell me about it?

Do not worry. All your questions should be answered on our Rho1D4-tag knowledge page.

I use the FLAG-tag (DYKDDDDK) for my protein purifications. Is it beneficial for me to switch to the Rho1D4-tag?

Yes, it definetly is! Have a look at this side-by-side comparison of the two.

Can I regenerate & reuse your Glutathione beads?

Yes, you can! Simply follow our protocol.