Rho Starter Set 3: PureCube Rho1D4 Cartridge (1x1 ml) + Rho1D4 peptide (5 mg)

€205.00 * €410.00 * (50% Saved)

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  • 33399
The decision to use a new affinity tag after working with another one for a long time can... more
Product information "Rho Starter Set 3: PureCube Rho1D4 Cartridge (1x1 ml) + Rho1D4 peptide (5 mg)"
Type: Cartridge
Bead size: 40 µm (Agarose)
Affinity : Rho1D4-tag
Purpose: Protein Purification
The decision to use a new affinity tag after working with another one for a long time can be an unappealing one. However the Rho1D4 tag offers numerous advantages over other antibody based affinity tags. See FLAG tag vs. Rho1D4 tag site as a prime example for this.
Cube Biotech offers a cost efficient option to quickly switch to the Rho1D4 tag. This option are our practical Rho1D4 Starter Sets.

The PureCube Rho1D4 Starter Set 3 is comprised of our PureCube Rho1D4 Cartridge with the Rho1D4 elution peptide for your finances & convenience.
rho1d4 tag membrane protein Schematic of a membrane protein tagged with Rho1D4

Features

 
Usage Highly specific affinity purification of Rho1D4 (TETSQVAPA) tagged proteins
Specifity Affinity to Rho1D4 tagged proteins
Special feature Highly recommended for the purification of membrane proteins.
Binding capacity 3-4 mg / ml Resin
Bead Ligand Rho1D4 Antibody
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Features - Regarding Cartridge / Column itself.

Feature PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Dimensions: diameter X length (mm) 6.2 x 50 11 x 80
Body material Polypropylene Acrylate
End Plug Material Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Max. Flow Rate 6 mL/min

Information about Rho1D4 elution peptide

Features

Usage Elution of Rho1D4 tagged proteins during the elution step of a protein purification procedure.
Specifity Affinity to Rho1D4 affinity beads
Sequence Ac_TETSQVAPA_ NH2
Amount 5 mg, lyophilized
Storage, short term Store lyophilized and reconstituted peptide at 4°C
Storage, long term Store lyophilized and reconstituted peptide at-20°C
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