SMALP 200
- Order number: 18210
Note: On October 1st 2021 this product was renamed from "SMALP 30010S" to "SMALP... more
Product information "SMALP 200"
Note: On October 1st 2021 this product was renamed from "SMALP 30010S" to "SMALP 200". Nothing else has changed. More on this renaming can be found here:
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins.
After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution.
After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use thethe Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution.
After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use thethe Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Features
| State | Lyophilized Powder, to be solved with water |
| Polymer styrene-to-maleic anhydride ratio | 2:1 |
| Molecular weight | 6.500 |
| pH after solving | 7.5 |
| Solubility | 40% |
| Divalent cationic tolerance | < 5 mM |
| Color | Yellowish |
| Odor | Odorless |
| Freeze-thaw stable? | Yes |
| Storage | 4°C for long-term |
| Other noteworthy feature | Most commonly used SMA |
| Structure |  |
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
Our protocol video that shows a full solubilization process using SMALP
Disclaimer
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