Sensory Rhodopsin-2 (Np)
Order number: 28931
Description
Sensory rhodopsin-2 (SR-2) from N. pharaonis. is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E.coli as a His-tagged protein.
Datasheets
Feature | |
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Alternative names | Sensory rhodopsin-2 (SR-2, NpSRII) |
UniProt Number | P42196 |
Protein class | 7-transmembrane protein |
Organism | Natronomonas pharaonis (Natronobacterium pharaonis) |
Sequence, explained | wild-type sequence, full-length, 7x His-tag |
Sequence, One-Letter Code | MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVADH HHHHHH |
Affinity tags | His-tag (C-terminus) |
Expression Host | E.coli |
Size | 246 amino acids; 26,315 Da |
Buffer composition | 4 M NaCl, 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM) |
Purity | >98%, determined via SDS-PAGE (Figure 1) |
Purified via | PureCube Ni-NTA Agarose |
Absorbance | Extinction coefficient at 500 nm: 45,500 M-1 cm-1.. Extinction coefficient at 590 nm: 54,000 M-1 cm-1. |
Activity | Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 500 nm = 2.8 |
Function | Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor |
Literature references |
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Lab Results
FAQ
Can I get the datasheet for the Sensory-Rhodopsin-2 (Np) protein?
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