Cubipol Fluorescein

Order number: 18731

€1,102.00*

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Description

Welcome the new addition to our NativeMP™ Platform: The Cubipol Series.

The proprietary Cubipol backbone, developed in-house, condenses the positive effects of previously available backbones like SMA, DIBMA, and UltrasoluteTM Amphipol. This new development delivers a new multimodal copolymer backbone, making the Cubipol series the first commercially available next-generation copolymer with 4 available off-the-shelf modified variants.
It joins the Next Generation Copolymers AASTY and Ultrasolute™ Amphipol, making solubilization and stabilization of membrane proteins a simpler and more effective endeavor than ever before.

Cubipol Fluorescein

This first-of-its-kind, functionalized version of our Cubipol is fluorescently labeled with fluorescein. This means a pH-dependent fluorescent excitation at ~485 nm and emission maximum at ~510 nm.

Suitable applications for fluorescent copolymers:
  • Detection
  • Localization (Visualization via fluorescent microscopy)
  • etc.

If you’re interested in having this functionalization applied to a different copolymer backbone, get in touch with us and we’ll get that project running for you: Contact us
Feature
State Lyophilized Powder, to be solved with water
Full Name Cubipol Fluorescein in 50 mM HEPES, pH 7.5
Buffer HEPES
pH after solving 7.5
pH Stability of Copolymer 5.0-10
Absorbance at 280nm Yes
Molecular Weight 7.4 kDA
Ca2+ tolerance 40 mM
Mg2+ tolerance 40 mM
Solubility > 10% (H2O)
Polymerization Type Free-radical
λAbs ~485 nm
λEm ~514 nm
Shipping Temperature Ambient Temparature
Storage of lyophilized copolymer -20°C for several years
Storage of dissolved copolymer 2-8°C for several days

Lab Results

Hydrpnamic Diameter of Cubipol Nanodiscs
Fig. 1: Hydrodynamic size of Cubipol-DMPC nanodiscs formed with different copolymers. Nanodisc size was measured in 150 mM NaCl, 20 mM HEPES at pH 7.5 and a copolymer/DMPC weigth ratio of 3/1.
Fig. 2: HPLC Analysis of Cubipol Fluoresceine Nanodiscs with a purified membrane protein – Eluates, Superose 6 Column, Emission at 510 nm, Excitation at 495 nm.
Cubipol Zeta-Potential
Fig. 3: ζ-potential of DMPC nanodiscs formed with different Cubipol copolymers. All four reveal negative net charge. With Glyco-Cubipol being the closest to neutrality at around -15 mV. Meanwhile unmodified Cubipol has the most negative net charge at -25 mV.
Fig. 4: HPLC Analysis of Cubipol Fluoresceine vs Cubipol showing an emission at 510 nm and excitation at 495 nm, 1% copolymer Solution in DMF.

Disclaimer

Patent Pending
The product described above has been developed and produced by Cube Biotech GmbH. Our products are intended for molecular applications. These products are not intended for diagnosis, prevention or treatment of a disease.