Sulfo-DIBMA

Order number: 18531

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Description

Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA) you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs in comparison usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Another major advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:

Datasheets

Features
Usage	Membrane protein solubilization & stabilization
Full-Name	Poly(diisobutylene-alt-N´,N´-/dimethyl(maleimidopropyl))ammonium propane sulfonate copolymer, sodium salt in 50 mM HEPES, pH 7.5
Formula Weight	8,000 g/mol ± 1000 g/mol
Buffer	50 mM HEPES
pH upon addition of dH₂O	7.5 ± 0.3
Total pH stability	4-10
State	Lyophilized Powder
Recommended concentration range upon use	1% to 5%
Solubility	> 10 % in H₂O
Absorbance at 280 nm	< 0.3 (1 % solution)
Mg²⁺ Tolerance	over 100 mM
Ca²⁺ Tolerance	over 100 mM
Color	White to slightly yellow
Odor	Odorless
Shipping Temperature	2-8°C
Long-term Storage (lyophilized copolymer)	-20°C for several years
Short-term Storage (dissolved copolymer)	2-8°C for several days

Lab Results

Sulfo-DIBMA chemical stucture — Fig. 1: The chemical structure of a Sulfo-DIBMA monomere.

Sulfo Dibma Westernblot — Fig. 2: Shown is the blot activity of a Rho-Tag purified human membrane protein expressed in HEK cells containing 9 transmembrane helices. The membrane protein was solubilized using Sulfo DIBMA as well as other polymers from the DIBMA family. Besides the efficient solubilization and purification of the membrane protein, the polymer belt of the resulting nanodiscs is electroneutral.

Sulfo Polymers Net Charge — Fig. 3: ζ-potential of DMPC nanodiscs formed with different polymers. Ultrasolute 18, DIBMA 10 and SMA 200 nanodiscs reveal a strong negative net charge whereas nanodiscs formed with Sulfo DIBMA and Sulfo SMA reveal a nearly electroneutral nanodisc compared to non-sulfonated controls. ζ-potential was measured in 150 mM NaCl, 20 mM HEPES at pH 7.5 and a Polymer/DMPC weigth ratio of 3/1.

Comparison of Hdrophobicity — Fig. 4: Hydrodynamic size of DMPC nanodiscs formed with different polymers. Nanodisc size was measured in 150 mM NaCl, 20 mM HEPES at pH 7.5 and a Polymer/DMPC weigth ratio of 3/1.

Sulfo Polymers CA 2+ Tolerance — Fig. 5: Ca²⁺ tolerance of Sulfo-Polymers. No polymer precipitation occurs up to 100 mM Ca²⁺ .

Sulfo Polymers MG 2+ Tolerance — Fig. 6: Mg²⁺ tolerance of Sulfo-Polymers. No polymer precipitation occurs up to 100 mM Mg²⁺.

Video

Watch our video on the use of synthetic polymers for membrane protein solubilization. It demonstrates the use with SMALP.

FAQ

Can I have the datasheet for Sulfo-DIBMA

Yes of course, you can download it here.

Is Sulfo-DIBMA from Cube Biotech ready to use?

Yes, our Sulfo-DIBMA is ready to use. You can start directly with the solubilization. Read our protocol for more information.

Which pH is suitable for Sulfo-DIBMA?

For Sulfo-DIBMA a pH of 7.5 is recommended. Sulfo-DIBMA does not solubilize if the pH is smaller than 6.5.

Which concentrations of Sulfo-DIBMA should I use for my protein?

In general, we advise you to add 2,5 % Sulfo-DIBMA to your solution. But the optimal conditions have to be screened by yourself. In general you should not go below 1% or over 5%. Please refer to our Membrane Protein Solubilization Protocol for more information. Or simply ask our support team.

Disclaimer

Patent Pending
The product described above is based on a substance published within the work of D. Glueck et al., Small, 2022, 2202492, DOI: 10.1002/smll.202202492.
Our products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.