Ultrasolute™ Amphipol 17

Ultrasolute™ Amphipol 17 by Cube Biotech, a cutting-edge synthetic copolymer specifically designed for membrane protein scientists. This highly purified copolymer, with a molecular weight (Mw) of approximately 7,300, is part of a novel series of developed copolymers for solubilization and stabilization of membrane proteins, introduced in 2022. It's important to note that Ultrasolute™ Amphipol 17, along with its counterpart Ultrasolute™ Amphipol 18, distinguishes itself from other amphipols available from different providers, as it possesses the unique ability to solubilize and stabilize membrane protein in one step, unlike older technologies lacking this capability.

Ultrasolute™ Amphipol 17 functions like a precision cookie cutter, extracting membrane proteins from the cell membrane while preserving the surrounding native phospholipids. The resulting nanodisc effectively makes the membrane protein into soluble in water, thanks to its hydrophobic surface facing the membrane phospholipids and a hydrophilic surface on the outer side. This distinctive configuration offers a compelling alternative to the conventional detergent-based approach for solubilizing membrane proteins.

Furthermore, Ultrasolute™ Amphipol 17 provides the secondary option of creating nanodiscs using an artificial phospholipid composition, similar to MSP nanodiscs. Demonstrated success has been achieved with phospholipids such as dimyristoyl-glycero-phosphocholine (DMPC) or palmitoyl-oleoyl-phosphatidyl-choline (POPC).

The resulting Ultrasolute™ Amphipol 17-nanodisc complex can be utilized in a multitude of biophysical assays, including SDS-PAGE, size exclusion chromatography (SEC), Western Blot, UV/Vis spectroscopy, and various chromatographic procedures, offering versatility and compatibility across a wide range of applications.

To assist researchers in finding the most suitable nanodisc copolymer for their specific membrane protein, Ultrasolute™ Amphipol 17 is part of our comprehensive Synthetic Nanodisc Screening Kit MAXI, which includes 17 different nanodisc copolymers in total.

The product is delivered as a lyophilized powder to be dissolved in ddH₂O. The targeted concentraion upon dissolving ranges between 0.1 and 2.5%, with 2.5% being the default concentration.