Nanodisc membrane scaffold proteins (MSPs)
Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, assembled nanodiscs are available.
Why Membrane scaffold proteins?
Variety of lyophilized membrane scaffold proteins available
Stable, lyophilized protein preparations in convenient aliquots
Can be combined with different phospholipids
Detailed protocols and expert support provided
MSP1D1-His and MSP1D1dH5-His were successfully used in the following publication:
Zeno, W.F. et al. Spectroscopic Characterization of Structural Changes in Membrane Scaffold Proteins
Entrapped within Mesoporous Silica Gel Monoliths.
Applied Materials & Interfaces 2015, 7, 8640-8649.