Nanodisc MSP proteins

Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of MSPs are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a His-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, assembled nanodiscs are available.

Why Membrane scaffold proteins?

Variety of lyophilized membrane scaffold proteins available
Stable, lyophilized protein preparations in convenient aliquots
Can be combined with different phospholipids
Also available untagged and as mouse/rat homolog proteins

Different MSP nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own distinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed down below, we also offer DIBMA and SMA based synthetic Nanodiscs.

Size

MSP1D1 ΔH5
Diameter
7-8 nm

MSP1D1
Diameter
9-10 nm

MSPE3D1
Diameter
12-14 nm

MSP2N2
Diameter
~ 17 nm

Species