Nanodisc membrane scaffold proteins (MSPs)
Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, assembled nanodiscs are available.
Why Membrane scaffold proteins?
- Variety of lyophilized membrane scaffold proteins available
- Stable, lyophilized protein preparations in convenient aliquots
- Can be combined with different phospholipids
- Detailed protocols and expert support provided
- Also available untagged and as mouse/rat homolog proteins
MSP1D1-His and MSP1D1dH5-His were successfully used in the following publication:
Zeno, W.F. et al. Spectroscopic Characterization of Structural Changes in Membrane Scaffold Proteins
Entrapped within Mesoporous Silica Gel Monoliths.
Applied Materials & Interfaces 2015, 7, 8640-8649.