MSP1E3D1, lyophilized protein

Order number: 26166

€1,463.00*

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Species

Human

Mouse

Amount

Quantity

Description

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1E3D1 creates nanodiscs of 12-14 nm in diameter.

This product is available with different species as its background. This is important for e.g. for secondary antibodies of a western blot.

Other MSP products or related sites by Cube Biotech include:

MSP nanodisc schematic overview — **Figure 1:** Schematic of a MSP nanodisc. A Membrane-scaffold-protein (MSP, green) forms a disc-shaped structure with some phospholipids.

Datasheets

Feature
Available origins	Human, mouse
Purity	> 90% (Determined by SDS-PAGE)
Number of amino acids	256 (Human origin) 254 (Mouse origin)
Molecular Mass	29,982 Da (Human origin) 29,786 (Mouse origin)
Extinction coefficient (in water) ε₂₈₀	26,930 M^-1cm^-1 (Human origin) 29,450 M^-1cm^-1 (Mouse origin)
Buffer	20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
Sequence (Human origin), for the other origins please send request	G STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
Affinity tag	none
Shipping Temperature	Room Temperature
Storage of lyophilized protein	-20°C for several months
Storage of reconstituted protein	2-8°C for several days
Helpful literature references	Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44 Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563 Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925

Citations

Stabilized Protein	Year	Author
ETB	2016	Rues R.-B., Henrich E., Boland C., Caffrey M., Bernhard F.6
DmelOR10a, DmelOR22a, DmelOR35a, and DmelOR71a	2019	Murugathas T., Zheng H. Y., Colbert D., Kralicek A., Carraher C., Plank N.
Type 1 IP3R	2021	Baker M.R.,Fan G., Seryshev A.B., Agosto M.A., Baker M.L., Serysheva I.I.
IP3R channel	2022	Fan G., Baker M.R., Terry L.E., Arige V., Chen M., Seryshev A.B., Baker M.L., Ludkte S.J., Yule D.I., Serysheva I.I.
MsbA	2024	Wang H., Ishchenko A., Skudlarek J., Shen P., Dzhekieva L., Painter R.E., Chen Y-T., Bukhtiyarova M., Leithead A., Tracy R., Babaoglu K., Bahnck-Teets C., Buevich A., Cabalu T.D., Labroli M., Lange H., Lei Y., Li W., Liu J., Mann P.A., Meng T., Mitchell H.J., Mulhearn J., Scaping G., Sha D., Shaw A.W., Si Q., Tong L., Wu C., Wu Z., Xiao J.C., Xu M., Zhang L-K., McKenney D., Miller R.R., Black T.A., Cooke A., Balibar C.J., Klein D.J., Raheem Z., Walker S.S.

Video

Watch our video tutorial about the creation of MSP nanodiscs. It explains the whole process, starting with the initial solubilzation using detergents. The protein that was stabilized in this demonstration was Bacteriorhodopsin.

We also recommend our video guide to nanodiscs in general.

FAQ

Can I get the datasheet for the MSP1E3D1 protein?

Yes. Just click here to download it as a PDF File.

Is this the correct MSP product for my membrane protein?

This cannot be answered easily as every membrane protein performs differently with each nanodisc size and phospholipid. It usually requires a screening process before. Remember that membrane proteins with fewer transmembrane domains (TMD) require a smaller nanodisc, while a high number of TMDs need a larger nanodisc.

Why are there different origins for the MSP protein and do they work differently?

First, no they all work the same. Do not worry. Second, the origin of the MSP protein is of importance e.g. western blots. As the secondary antibody is usually against a species e.g anti-mouse. To avoid unwanted cross reactions the origin of the MSP protein must be known.

I have never worked with MSP nanodiscs before. Can you tell me more about them?

Of course! We created THIS MSP NANODISC GUIDE PAGE, for this purpose. Have a look at it!

Are there other options to stabilize membrane proteins besides MSP nanodiscs?

Yes, there are. We recommend synthetic copolymer nanodiscs greatly! Have a look at THIS GUIDE PAGE to learn more.