MSP2N2-His, lyophilized protein

Order number: 26172

€215.00*

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Species

Human

Mouse

Amount

Quantity

Description

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP2N2 (His-tagged) creates nanodiscs of ~17 nm in diameter.

Note that this products contains a N-terminal His-tag. Keep that in mind when chosing the affinity tag for your membrane protein.

Other MSP products or related sites by Cube Biotech include:

MSP nanodisc schematic overview — **Figure 1:** Schematic of a MSP nanodisc. A Membrane-scaffold-protein (MSP, green) forms a disc-shaped structure with some phospholipids.

Datasheets

Feature
Available origins	Human, mouse
Purity	> 90% (Determined by SDS-PAGE)
Number of amino acids	392 (Human origin) 388 (mouse origin)
Molecular Mass	45,670 Da (Human origin) 45,526 Da (Mouse origin)
Extinction coefficient (in water) ε₂₈₀	44,920 M^-1cm^-1 (Human origin) 39,880 M^-1cm^-1 (Mouse origin)
Buffer	20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
Sequence (Human origin), for the other origins please send request	GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ GT PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
Affinity tag	N-Terminal His-tag
Shipping Temperature	Room Temperature
Storage of lyophilized protein	-20°C for several months
Storage of reconstituted protein	2-8°C for several days
Helpful literature references	Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44 Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563 Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925

Citations

Stabilized Protein	Year	Author
Look up in publication	2021	Nibali S.C., Di Rosa M.C., Rauh O., Thiel G., Reina S., De Pinto, V.

Video

Watch our video tutorial about the creation of MSP nanodiscs. It explains the whole process, starting with the initial solubilzation using detergents. The protein that was stabilized in this demonstration was Bacteriorhodopsin.

We also recommend our video guide to nanodiscs in general.

FAQ

Can I get the datasheet for the MSP2N2 (His-tagged) protein?

Yes. Just download them here.

Is this the correct MSP product for my membrane protein?

This cannot be answered easily as every membrane protein performs differently with each nanodisc size and phospholipid. It usually requires a screening process before. Remember that membrane proteins with fewer transmembrane domains (TMD) require a smaller nanodisc, while a high number of TMDs need a larger nanodisc.

I have never worked with MSP nanodiscs before. Can you tell me more about them?

Of course! In fact we created THIS MSP NANODISC GUIDE PAGE, for this purpose. Have a look at it!

Are there other options to stabilize membrane proteins besides MSP nanodiscs?

Yes, there are. We recommend synthetic copolmer nanodiscs greatly! Have a look at THIS GUIDE PAGE to learn more.