eGFP (His-tag) Protein

Order number: 29901

€261.00*

Ready to ship today
Quantity

Description

The discovery of Green Fluorescent Protein (GFP) is one of the most influential proteins in modern biology. Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection. Purified eGFP can find multiple uses in scientific research. Often as positive controls for protein purifications or western blots. Therefore we offer eGFP tagged with the most commonly used affinity tags for these purposes.

We offer eGFP with the following affinity-tag combinations
  • N-Terminal His-tag
  • N-Terminal GST-tag and C-Terminal Rho1D4-tag
  • N-Terminal GST-tag and C-Terminal DYKDDDDK-tag
Feature - eGFP (His-tag)
Protein class Beta-barrel; Autofluorescent Protein
Sequence - Sections named from N-Ter to C-Ter His-tag, eGFP
Sequence - Amino Acids (1-Letter-Code) N-terminus-
MKHHHHHHHMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHNVYITA
DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK
DPNEKRDHMVLLEFVTAAGITHGMDELYK-C-terminus
Affinity tags His-tag (N-Terminus)
Size (kDA) 26 kDA
Size (Amino Acid count) 249
Excitation maximum 488 nm
Fluorescence maximum 509 nm
Purity >98% as determined by SDS-PAGE


Feature - eGFP (GST- and Rho1D4-tag)
Protein class Beta-barrel; Autofluorescent Protein
Sequence - Sections named from N-Ter to C-Tery GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP, Spacer, Rho1D4-tag
Sequence - Amino Acids (1-Letter-Code) N-terminus-
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNL
PYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIA
YSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM
DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD
LVPRGSLEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY
GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQ
ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ
SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGTETSQVAPA-C-terminus
Affinity tags GST-tag (N-Terminus), Rho1D4-tag (C-terminus)
Size (kDA) 55.4 kDA
Size (Amino Acid count) 488
Excitation maximum 488 nm
Fluorescence maximum 509 nm
Purity >98% as determined by SDS-PAGE


Feature - eGFP (GST- and DYKDDDDK-tag)
Protein class Beta-barrel; Autofluorescent Protein
Sequence - Sections named from N-Ter to C-Tery GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP Spacer, DYKDDDDK-tag
Sequence - Amino Acids (1-Letter-Code) N-terminus-
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNL
PYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIA
YSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM
DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD
LVPRGSLEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY
GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQ
ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ
SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGDYKDDDDK-C-terminus
Affinity tags GST-tag (N-Terminus), Rho1D4-tag (C-terminus)
Size (kDA) 55.5 kDA
Size (Amino Acid count) 486
Excitation maximum 488 nm
Fluorescence maximum 509 nm
Purity >98% as determined by SDS-PAGE

Lab Results

All of our eGFPs are purified to the highest degree. We use them ourselves for the quality control of our matching protein purification beads.
eGFP westernblots
Figure 1: Size and purity of three eGFP variants assessed by SDS-PAGE.