eGFP (His-tag) Protein
Order number: 29901
Description
The discovery of Green Fluorescent Protein (GFP) is one of the most influential proteins in modern biology. Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection. Purified eGFP can find multiple uses in scientific research. Often as positive controls for protein purifications or western blots. Therefore we offer eGFP tagged with the most commonly used affinity tags for these purposes.
We offer eGFP with the following affinity-tag combinations
We offer eGFP with the following affinity-tag combinations
- N-Terminal His-tag
- N-Terminal GST-tag and C-Terminal Rho1D4-tag
- N-Terminal GST-tag and C-Terminal DYKDDDDK-tag
Datasheets
Feature - eGFP (His-tag) | |
---|---|
Protein class | Beta-barrel; Autofluorescent Protein |
Sequence - Sections named from N-Ter to C-Ter | His-tag, eGFP |
Sequence - Amino Acids (1-Letter-Code) | N-terminus- MKHHHHHHHMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHNVYITA DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK DPNEKRDHMVLLEFVTAAGITHGMDELYK-C-terminus |
Affinity tags | His-tag (N-Terminus) |
Size (kDA) | 26 kDA |
Size (Amino Acid count) | 249 |
Excitation maximum | 488 nm |
Fluorescence maximum | 509 nm |
Purity | >98% as determined by SDS-PAGE |
Feature - eGFP (GST- and Rho1D4-tag) | |
---|---|
Protein class | Beta-barrel; Autofluorescent Protein |
Sequence - Sections named from N-Ter to C-Tery | GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP, Spacer, Rho1D4-tag |
Sequence - Amino Acids (1-Letter-Code) | N-terminus- MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNL PYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIA YSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD LVPRGSLEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQ ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHN VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGTETSQVAPA-C-terminus |
Affinity tags | GST-tag (N-Terminus), Rho1D4-tag (C-terminus) |
Size (kDA) | 55.4 kDA |
Size (Amino Acid count) | 488 |
Excitation maximum | 488 nm |
Fluorescence maximum | 509 nm |
Purity | >98% as determined by SDS-PAGE |
Feature - eGFP (GST- and DYKDDDDK-tag) | |
---|---|
Protein class | Beta-barrel; Autofluorescent Protein |
Sequence - Sections named from N-Ter to C-Tery | GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP Spacer, DYKDDDDK-tag |
Sequence - Amino Acids (1-Letter-Code) | N-terminus- MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNL PYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIA YSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD LVPRGSLEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQ ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHN VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGDYKDDDDK-C-terminus |
Affinity tags | GST-tag (N-Terminus), Rho1D4-tag (C-terminus) |
Size (kDA) | 55.5 kDA |
Size (Amino Acid count) | 486 |
Excitation maximum | 488 nm |
Fluorescence maximum | 509 nm |
Purity | >98% as determined by SDS-PAGE |
Lab Results
All of our eGFPs are purified to the highest degree. We use them ourselves for the quality control of our matching protein purification beads.
![eGFP westernblots](/media/77/7e/c5/1668427522/Three eGFP purified variants.jpg)