
- Order number: 29921
Every well-performed experiment needs a positive and negativ controll during its run. THis of... more
Product information "GST-eGFP fusion protein, DYKDDDDK-tag"
Every well-performed experiment needs a positive and negativ controll during its run. THis of course also applies to protein purifications processes. For this purpose we created this GST-eGFP fusion protein, that is tagged with the commonly used FLAG-tag. Therefore, it contains multiple affinity tags and can therefore be used as a positive control for different purification assays. It does contain:
- N-Terminal GFP
- Multiple cleavage sites
- GST-tag, meant for our GST-tag protein purification products
- C-Terminal DYKDDDDK-tag
Features
Protein class | Beta-barrel; Autofluorescent Protein |
Sequence - Sections named from N-Ter to C-Ter | GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP Spacer, DYKDDDDK-tag |
Sequence - Amino Acids | N-terminus-MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQS MAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLY DALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSLVPRGS LEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNGHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGDYKDDDDK-C-Terminus |
Affinity tags | GFP-tag (N-terminus), GST-tag (Middle), FLAG / DYKDDDDK-tag (C-terminus) |
Size (kDA) | 55.5 kDA |
Size (Amino Acid count) | 486 amino acids |
Excitation maximum | 488 nm |
Fluorescence maximum | 509 nm |
Purity | >98% as determined by SDS-PAGE (see Datasheet) |
Related links to "GST-eGFP fusion protein, DYKDDDDK-tag"
Viewed