PureCube Glutathione Cartridges and Compact Cartridges
The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag, Strep tag, or Rho1D4 tag, and is often chosen to promote recombinant protein folding. Cube Biotech offers high-performance PureCube Glutathione Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).
PureCube Glutathione Cartridges provide:
Fig. 1: Purification yield is comparable to that of a market-leading resin.SDS-PAGE of GST expressed in E.coli and purified in gravity columns with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Up to 10 mg/ml protein yield was obtained with PureCube Glutathione Agarose (elution fractions E1-E6, Cube). TL: total lysate; CL: cleared lysate; FT: flow-through; W1/W4: wash fractions; E1-E6: elution fractions.
High protein binding capacity
In a comparative study with equivalent, market-leading resins, PureCube Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Figure 1 is an SDS-PAGE of GST purified on gravity columns packed with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Both resins yielded up to 10 mg/mL protein.