GST Affinity Cartridges


FPLC Columns
The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag, Strep tag, or Rho1D4 tag, and is often chosen to promote recombinant protein folding. Cube Biotech offers high-performance PureCube Glutathione Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube Glutathione Cartridges provide:


GST-tagged protein purification with PureCube Glutathione Agarose
Fig. 1: Purification yield is comparable to that of a market-leading resin.SDS-PAGE of GST expressed in E.coli and purified in gravity columns with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Up to 10 mg/ml protein yield was obtained with PureCube Glutathione Agarose (elution fractions E1-E6, Cube). TL: total lysate; CL: cleared lysate; FT: flow-through; W1/W4: wash fractions; E1-E6: elution fractions.
High protein binding capacity
In a comparative study with equivalent, market-leading resins, PureCube Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Figure 1 is an SDS-PAGE of GST purified on gravity columns packed with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Both resins yielded up to 10 mg/mL protein.

Features

Feature PureCube GST affinity Cartridge, 1 & 5 mL Purecube GST affinity COMPACT Cartridge, 1& 5 mL
Usage Purification of GST tagges proteins
Specifity Affinity to GST tagged proteins
Technology IMAC
Protein Binding capacity
10 mg (1ml cartridge)
50 mg (5ml cartridge)
pH stability 3-12
Other Compatibilities 1 M sodium acetate, pH 4.0, or 6 M guanidinehydrochloride, organic solvents (e.g., 70% (v/v) ethanol), 1% (w/v) SDS, 0.1 M NaOH, 0.1 M HCl.
Bead size ~40 μm
Storage temperature 2-8 °C
Functional Group Anti-GST antibody
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 7.5% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
FPLC Columns
The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag, Strep tag, or Rho1D4 tag, and is often chosen to promote recombinant protein folding. Cube Biotech offers high-performance PureCube Glutathione Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube Glutathione Cartridges provide:


GST-tagged protein purification with PureCube Glutathione Agarose
Fig. 1: Purification yield is comparable to that of a market-leading resin.SDS-PAGE of GST expressed in E.coli and purified in gravity columns with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Up to 10 mg/ml protein yield was obtained with PureCube Glutathione Agarose (elution fractions E1-E6, Cube). TL: total lysate; CL: cleared lysate; FT: flow-through; W1/W4: wash fractions; E1-E6: elution fractions.
High protein binding capacity
In a comparative study with equivalent, market-leading resins, PureCube Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Figure 1 is an SDS-PAGE of GST purified on gravity columns packed with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Both resins yielded up to 10 mg/mL protein.

Features

Feature PureCube GST affinity Cartridge, 1 & 5 mL Purecube GST affinity COMPACT Cartridge, 1& 5 mL
Usage Purification of GST tagges proteins
Specifity Affinity to GST tagged proteins
Technology IMAC
Protein Binding capacity
10 mg (1ml cartridge)
50 mg (5ml cartridge)
pH stability 3-12
Other Compatibilities 1 M sodium acetate, pH 4.0, or 6 M guanidinehydrochloride, organic solvents (e.g., 70% (v/v) ethanol), 1% (w/v) SDS, 0.1 M NaOH, 0.1 M HCl.
Bead size ~40 μm
Storage temperature 2-8 °C
Functional Group Anti-GST antibody
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 7.5% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
Top seller
GST Affinity Cartridges
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PureCube Glutathione Cartridge PureCube Glutathione Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml Glutathione Agarose
Article number: 32301
Sales price: From €39.00 *
PureCube Compact Cartridge Glutathione PureCube Compact Cartridge Glutathione
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml Glutathione Agarose
Article number: 32302
Sales price: From €39.00 *
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