PureCube Glutathione Agarose

Order number: 32103

€190.00*

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Description

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. This is why GST-tag purifications exploit this specificity to gain high levels of purity while maintaining a high protein yield.

PureCube Glutathione Agarose is a suspension of small 40 µm purification beads that are used for this purpose. Their small size provides high mechanical stability. They can be used for all sorts of affinity chromatography methods, such as FPLC, batch spin, or column chromatography.

You can also purchase these agarose beads pre-packed into FPLC-ready cartridges. Additionally, we offer GST-tag affinity resin with a bead diameter of 400 µm for very viscous cell media and higher FPLC flow rates. Alternatively, Glutathione MagBeads are also available.

Additional support products for GST-tag purification assays are L-Glutathione (reduced), an essential compound of the elution buffer, and our GST-tag antibody for later western blot detection of your protein.
Feature
Usage Specific binding and purification of GST-tagged proteins
Specificity Affinity to GST(Gluthathione-S-Transferase)-tagged proteins
Binding capacity >10 mg/ml
Bead Ligand L-Glutathione (reduced)
Bead size 40 μm
Matrix 7.5% cross-linked agarose
Filling quantity Delivered as a 50 % suspension
pH Stability 3-12
Required equipment
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Lab Results

Lab funds are sadly often the limiting factor when it comes to research. So it is of utmost importance that one gets the most value from every purchase one does. The reusability of products and materials is a key word here!

Our GST-tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST-tag purification with the same beads is ensured to be of the same high quality.
Performance of PureCube Glutathione Purification beads
Figure 1: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.

FAQ

Can I get the datasheet for the PureCube Glutathione Agarose?

Why are there beads of different sizes for the same purpose?

It is a balance to strike here between protein yield (smaller beads) and other factors like higher FPLC flow rates or handling of very viscous cell media (bigger beads).

Can I regenerate & reuse your Glutathione beads?