PureCube IDA magnetic beads wwew developed for IMAC based purification and enrichment methods. Since the affinity of an IDA-metal-ion-complex depends on the loaded metal ion, we offer IDA MagBeads uncoupled to any metal ion for the case that we do not offer your desired IDA + metal ion combination. By providing our coupling protocols, feel free to create any IDA purification resin you desire. PureCube IDA MagBeads XL are also available regular sized for higher protein purifications of more viscous cell lysates or as IDA agarose resin. Furthermore PureCube IDA agarose can also be obtained pre-filled into FPLC cartridges / columns.
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Features
Usage
For coupling of metal ions for corresponding purification and / or enrichment purposes
Specifity
Varies
Binding capacity
Depends on the loaded ion
Purity
Depends on the loaded ion
Bead Ligand
IDA
Bead size
90 μm
Matrix
6% cross-linked agarose
Recommended FPLC flow rates
0.5-2 mL/min
Maximal FPLC flow rate
6 mL/min
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
