PureCube Glutathione Agarose

PureCube Glutathione Agarose
€164.00 *

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mL:

  • 32103
PureCube GST Agarose resin exploits the enzyme-substrate specificity of the... more
Product information "PureCube Glutathione Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : GST-tag
Purpose: Protein Purification
PureCube GST Agarose resin exploits the enzyme-substrate specificity of the Glutathione-S-Transferase (GST) to its substrate reduced L-Glutathione for protein purification. PureCube GST Agarose Resin beads carry L-Glutathione, while the protein of interest is tagged with GST (26 kDa). This allows for incredible specificty and therefore highly pure protein. These agarose beads have a diameter of 40 µm. and can be used for batch spin columns and FPLC based purification assays.
Alternatively we offer Glutathione XL agarose beads for higher FPLC flow rates and GST Affinity Magnetic Beads
Futhermore PureCube Gluathione Agarose can be purchased prepacked in our PureCube Gluthathione Cartridges

Features

Usage Specific binding and purification of GST-tagged proteins
Specifity Affinity to GST(Gluthathione-S-Transferase)-tagged proteins
Binding capacity >10 mg/mL
Bead Ligand L-Glutathione (reduced)
Bead size Ø 40 µm
Stabilities 1 M sodium acetate, pH 4.0, or 6 M guanidinehydrochloride, organic solvents (e.g., 70% (v/v) ethanol), 1% (w/v) SDS, 0.1 M NaOH, 0.1 M HCl.
pH Stability 3-12
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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