PureCube Octyl Agarose
- Order number: 39203
Our PureCube Phenyl Agarose has been synthesized for hydrophobic interaction chromatography... more
Product information "PureCube Octyl Agarose"
| Type: | Agarose |
| Bead size: | 40 µm (Agarose) |
| Affinity : | Hydrophobic Interaction |
| Purpose: | Protein Purification |
Our PureCube Phenyl Agarose has been synthesized for hydrophobic interaction chromatography (HIC) based protein purification assays. Usually high salt concentration achieved are required for protein bindung, while elution is performed with a decreasing salt gradient.
If you purify a protein for the first time using HIC it is required in the beginning to screen for the best HIC agarose resin. They differ from protein to protein in term of protein affinity and protein yield. For this screening purpose we also offer additional HIC agarose resins, namely PureCube Butyl Agarose and PureCube Phenyl Agarose.
All three of our PureCube HIC agarose resins (Butyl, Phenyl and Octyl-) can be purchased together in our practical PureCube HIC Starter Set.
If you purify a protein for the first time using HIC it is required in the beginning to screen for the best HIC agarose resin. They differ from protein to protein in term of protein affinity and protein yield. For this screening purpose we also offer additional HIC agarose resins, namely PureCube Butyl Agarose and PureCube Phenyl Agarose.
All three of our PureCube HIC agarose resins (Butyl, Phenyl and Octyl-) can be purchased together in our practical PureCube HIC Starter Set.
Schematic of a Octyl groupFeatures
| Usage | Protein Purification using hydrophobic interactions |
| Bead Ligand | Covalently coupled octyl group |
| Butyl group density | 40 µmol/ml |
| Recommended FPLC flow rates | 0.5 -2 ml/min |
| Maximal FPLC flow rates | 6 ml/min |
| Matrix | 7.5% cross-linked agarose |
| Bead size | 40 μm |
| pH stability | 2-13 |
| Filling quantity | Delivered as a 50 % suspension |
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