PureCube Butyl Agarose
Order number: 39003
Description
Hydrophobic interaction chromatography (HIC) is a reliable method to separate proteins and other biomolecules based on their hydrophobicity. Our PureCube Butyl agarose resin is a suspension of purification beads for such an approach. If you use HIC with your sample for the first time we recommend testing all of our HIC resins before.
These include:
These include:
| Feature | |
|---|---|
| Usage | As a matriy for Hydrophobic Interaction Chromatography (HIC) |
| Specificity | Hydrophobic biomolecules and proteins |
| Bead size | 100 μm |
| Bead Ligand | Butyl groups |
| Butyl group density | 40 µmol/ml |
| BSA binding capacity | 25 mg/ml |
| lysozyme binding capacity | 45 mg/ml |
| pH stability | 2-13 |
| Filling quantity | Delivered as a 50 % suspension |
| Matrix | 7.5% cross-linked agarose |
| Short Term Storage | In neutral buffer at 4°C |
| Long Term Storage | 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C |
| Chemical Structure of Butyl ligand |  |
Video
Video Guide - Hydrophobic Interaction Chromatography (Jump to 8:04)
Video Guide - How to pack FPLC cartridges
FAQ
Can I get the datasheet for the PureCube Butyl Agarose?
What can I do with these beads?
The beads are meant for Hydrophobic Interaction Chromatography or HIC for short. This purification method separates proteins and other biomolecules based on their hydrophobicity. How the process works in detail can be found in THIS VIDEO GUIDE