Rho Starter Set 3: HighSpec Compact Cartridge Rho1D4 (1x1 ml) + HighSpec Rho1D4 peptide (5 mg)
Order number: 33398
Description
Our Rho1D4 Starter Set 3 is a combination of our HighSpec Rho1D4 Compact Cartridge and the Rho1D4 elution peptide.
The Compact Cartridge itself is an FPLC column pre-packed with our HighSpec Rho1D4 agarose resin.
It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. More on the Rho1D4-tag can be read here.
Do you prefer your agarose resin unpacked for e.g. column purification or magnetic beads, as we also offer other Rho1D4 Starter Sets.
Do you prefer your agarose resin unpacked for e.g. column purification or magnetic beads, as we also offer other Rho1D4 Starter Sets.
- Starter Set 1: Rho1D4 Agarose + 5 mg Rho1D4 peptide
- Starter Set 2: Rho1D4 MagBeads + 5 mg Rho1D4 peptide
Datasheets
Feature - Pre-packed Rho1D4 Agarose | |
---|---|
Usage | Specific binding and purification of Rho1D4-tagged proteins |
Specificity | Affinity to Rho1D4-tagged (TETSQVAPA) proteins |
Binding capacity | >3 mg/ml |
Bead Ligand | anti-Rho1D4 antibody |
Bead size | 40 μm |
Matrix | 7.5% cross-linked agarose |
Recommended flow rates | 0.25 - 1 ml/min |
Max. flow rates | 6 ml/min |
Filling quantity | Delivered as a 50 % suspension |
Short-term storage | In neutral buffer at 4°C |
Long-term storage | In neutral buffer with 20% ethanol at 4°C |
Required equipment & materials |
|
Feature - Column | ||
---|---|---|
PureCube Compact Cartridge 1 mL | PureCube Compact Cartridge 5 mL | |
Bed Volume | 1 mL | 5 mL |
Max. Flow Rate | 6 mL/min | 6 mL/min |
Dimensions: diameter X length (mm) | 5 X 35 | 17 X 35 |
Body material | Polypropylene | Polypropylene |
Inlet | 10-32 UNF female thread | 10-32 UNF female thread |
Outlet | 10-32 UNF female thread | 10-32 UNF female thread |
Feature - Rho1D4 Elution Peptide | |
---|---|
Usage | Elution of Rho1D4-tagged protein from matching purification beads |
Specifity | Affinity to Rho1D4 affinity beads |
Sequence | Ac_TETSQVAPA_NH2 |
Amount | 5 mg per tube |
Storage, short term | Store lyophilized and reconstituted peptide at 4°C |
Storage, long term | Store lyophilized and reconstituted peptide at-20°C |
Lab Results
Rho1D4-tag purification products are especially handy when it comes to the purification of proteins with low abundance. And a huge group of these proteins is membrane proteins. Even overexpressed their concentration does not come close to their soluble counterparts.
Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
FAQ
Can I get the datasheet for the HighSpec Rho1D4 Compact Cartridge or Rho1D4 peptide?
Can I use the Rho1D4-tag also for non-membrane proteins?
Yes, you can.
I never heard about the Rho1D4-tag before. What can you tell me about it?
Do not worry. All your questions should be answered on our Rho1D4-tag knowledge page.
I use the FLAG-tag (DYKDDDDK) for my protein purifications. Is it beneficial for me to switch to the Rho1D4-tag?
Yes, it definetly is! Have a look at this side-by-side comparison of the two.