
- Order number: 29911
Every valid experiments needs positive and negative controlls during its run. So do protein... more
Product information "GST-eGFP fusion protein, Rho1D4-tag"
Every valid experiments needs positive and negative controlls during its run. So do protein purifications. For this purpose we created this GST-eGFP fusion protein. It contains multiple affinity tags and can therefore be used as a positive control for multiple different purification assays. It does contain:
- N-Terminal GFP
- Multiple cleavage sites
- GST-tag, meant for our GST-tag protein purification products
- C-Terminal Rho1D4-tag, meant for our Rho1D4-tag protein purification products
Features
Protein class | Beta-barrel; Autofluorescent Protein |
Sequence - Sections named from N-Ter to C-Ter | GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP, Spacer, Rho1D4-tag |
Sequence - Amino Acids | N-terminus-MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQS MAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLY DALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGS LEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNGHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGTETSQVAPA-C-Terminus |
Affinity tags | GFP-tag (N-terminus), GST-tag (Middle), Rho1D4-tag (C-terminus) |
Size (kDA) | 55.4 kDA |
Size (Amino Acid count) | 487 amino acids |
Excitation maximum | 488 nm |
Fluorescence maximum | 509 nm |
Purity | >98% as determined by SDS-PAGE (see Datasheet) |
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